The tests that have been previously described (p. 105) have been devised especially for testing the quality of the milk for cheese making purposes, and are of the greatest service to the maker in tracing the source of poor milk.

Cheddar cheese. The first step in the making of cheddar cheese is the "ripening" of the milk, or the development of a small amount of acid. In this fermentation, the development of acid is preceded by an enormous increase in the number of acid-forming bacteria. Milk for cheese making should show an acidity of about 0.2 per cent or slightly more than in fresh milk. In other words, the maker wishes the milk to be in such condition, bacteriologically, that if kept at a temperature favorable for the growth of the acid-forming bacteria, the acidity will increase rapidly.

The curdling of the milk to precipitate the cheese solids is produced by the addition of rennet, which is obtained by extracting the fourth stomach of the young calf with a solution of common salt. In the past the maker prepared his own rennet solution from the dried stomachs ("rennets"), but at present, the extract is prepared commercially, in a much more uniform manner. The rapidity of the curdling is dependent upon the acidity of the milk. In order to secure proper rennet action, a slight increase of acid over that found in fresh milk is usually necessary; thus at the very beginning of the process of making cheddar cheese, the bacteria are of importance.

As the milk curdles, the bacteria are enclosed in the curd as are the fat globules. The curd is cut into small fragments by means of a curd knife, and as the mass is warmed, the acid develops, causing the curd particles to shrink, thus expressing the whey. Within a short time, the volume of the curd is not more than one-eighth that of the milk, but in the curd are held over 75 per cent of the bacteria of the milk. To secure rapid curdling in the vat, the milk is warmed to 85° to 90° F., a temperature that is most favorable for the growth of the lactic bacteria. Since there is a large number of bacteria concentrated in a small volume, and the temperature, as well as all other conditions, is favorable to growth, multiplication of the bacteria goes on rapidly, and as a consequence, acid is formed in large amounts, as is shown by the following figures given by Publow for the manufacture of the export type of cheddar cheese:

Acidity of milk before adding rennet.2 to .21per cent
Acidity of whey before heating curd.14 to .145"
Acidity of whey before removing from curd.16 to .18"
Acidity of whey coming from the curd after removal of whey and curd is packed.24 to .30"
Acidity of whey coming from curd before milling.65 to .75"
Acidity of whey coming from curd before salting.90 to 1.10"

If the milk had been kept at the same temperature as the curd, the acidity would have increased much more slowly since the acid would have been distributed through a larger volume. In the cheese curd the same amount of acid is probably formed, as would have been produced in the total amount of milk during the same interval.

The acid produced by this bacterial activity has a most marked effect on the curd. At first the curd masses are tough and firm, the particles showing no tendency to adhere to each other. As the acid increases in amount, the curd becomes plastic, the outer surface of the particles adhering or "matting," as the maker expresses it. The result is a solid coalescent mass of curd, which is cut into small pieces, i.e., "milled," before it is put to press. The acid allows the blending of the pieces under the influence of the pressure so that a cheese is one single mass. Under certain abnormal conditions, the development of acid may be interfered with and the particles of curd fail to mat, in which case, the cheese will be crumbly when it is cut. The determination of the proper time for pressing is made by the application of what is known as the hot iron test. This is made by determining the length of the "strings" or "threads" which can be drawn from a mass of curd when it is brought in contact with a hot iron at a cherry red heat, the length of the curd threads being a measure of the amount of acid that has been formed in the curd.

The rate of acid formation within the curd particles is also measured by determining the acidity of the whey as it comes from the curd at different stages in the making. This test, which is often used in place of the "hot iron" test is carried out in the same manner, as in determining the acidity of milk or cream. The quality of the cheese, both as to texture and flavor, is dependent to a great degree upon the amount of acid that is formed during the various stages in making; hence, the successful maker must follow closely by some means the acid formation in the curd until it is put to press.

It is very necessary that the milk shall contain a sufficient number of acid-forming bacteria to produce the required amount of acid. If a sufficient number of bacteria are not present in the milk as it is received, as is the case with very sweet milk, they must be added by the maker in the form of a starter, or the process of making will be much prolonged.