Table III.—Effect of different types of artificial manipulation on the seed production of sweet clover at Arlington, Va., and at Ames, Iowa, in 1916.
| Location. | Experiment. | Total number of— | Flowers that set seed (per cent). | ||||
| Racemes. | Flowers. | Pods set. | At each station. | Average. | |||
| Arlington | A | 49 | 3,510 | 144 | 4.1 | } | 2.9 |
| Ames | A | 84 | 4,536 | 92 | 2.0 | ||
| Arlington | B | 100 | 5,599 | 3,973 | 70.95 | } | 66.51 |
| Ames | B | 196 | 1,276 | 600 | 47.02 | ||
| Arlington | C | 50 | 1,229 | 701 | 57.03 | } | 54.94 |
| Ames | C | 75 | 289 | 133 | 46.02 | ||
| Arlington | D | 50 | 1,480 | 936 | 63.24 | } | 62.18 |
| Ames | D | 88 | 575 | 342 | 59.47 | ||
| Arlington | E | 31 | 377 | 307 | 81.43 | } | 70.10 |
| Ames | E | 48 | 175 | 80 | 45.71 | ||
| Arlington | F | 30 | 933 | 524 | 56.16 | ......... | |
SEED PRODUCTION OF MELILOTUS ALBA UNDER ORDINARY FIELD CONDITIONS.
The production of seed of Melilotus alba under ordinary field conditions varies considerably, not only in different parts of the country but also on different fields in the same region. A number of factors contribute to this variation, one of the most important of which appears to be the inability of the plant to supply all the developing seed with sufficient moisture, causing some of them to abort. As pointed out on page 22 this condition was very marked in certain parts of the country in 1916. However, poor seed production is not always correlated with lack of moisture, for the seed crop was a failure in 1915, where cloudy and rainy weather prevailed much of the time the plants were in bloom. It is believed that the lack of pollination by insects was the principal cause for the failure of seed to set, as very few insects visit sweet-clover flowers when such conditions prevail. As sweet-clover pollen will germinate in pure water and as plants which have their roots submerged in water set seed abundantly when pollinated, the failure of the seed crop in 1915 was not due to excessive moisture.
As a rule, thin stands of sweet clover produce more seed to the acre than thick stands and isolated plants more seed than those growing in either a thick or thin stand. The correlation of seed production with the thickness of stand is probably due to the shading and partial prevention of insect visitation to part of the racemes on the lower branches. Most of the flowers upon the lower branches of isolated plants are directly exposed to sunlight and to insect visits: therefore the racemes on these branches produce as large a percentage of seed as the racemes on the upper branches. In a thick stand, little seed is produced by racemes on the lower branches.
A plant approximately 3 feet high growing close to the center of a field at Arlington. Va., in which was an average stand of four sweet-clover plants to the square foot was selected in order to determine the number of racemes produced and the average number of seeds to the raceme. This plant produced 196 racemes, which contained an average of 20.4 pods each. The racemes varied from 2 to 10 cm. in length, and the number of pods to the raceme ranged from to 75. The racemes on the upper and most exposed portions of the plants were larger and the flowers produced a much higher percentage of pods than the racemes close to the bases of the larger branches. Many of the small racemes on the lower branches produced less than five pods each.
The data obtained from the two plants at Arlington that were protected from night-flying insects may also be cited here, as the results of that experiment show that night-flying insects are not an important factor in the production of sweet-clover seed, and, further. because they were growing under the same conditions, in the same plat, and were approximately of the same size. These two plants produced a total of 544 racemes, with an average of 20.9 pods each. The number of pods to the raceme varied from to 86.
EFFICIENCY OF CERTAIN KINDS OF INSECTS AS POLLINATORS OF SWEET CLOVER.
In order further to test the self-sterility of sweet clover and to determine the relative efficiency of night-flying and of different kinds of day-flying insects as pollinators of the flowers, a number of cages covered with cheesecloth, glass, or wire screen having 14 meshes to the linear inch were placed over plants at Arlington. Va., and at Ames. Iowa, in July and August. 1916. The bases of the cages were buried several inches in the ground, so that insects could not pass under them. Cheesecloth was used to cover most of the cages and was made into sacks of such a size that they could be put on or removed from the frames of the cages without difficulty. It was stretched tightly over the frames and fastened to their bases with laths.
A cage having two sides and the top of glass but with ends covered with cheesecloth to permit ventilation was used at Ames to protect a number of plants from insect visitation at all times. The purpose of this cage was to determine whether the partial shading of the plants in the cages covered with cheesecloth would have any effect upon the setting of seed.