Excepting soil acidity, lack of inoculation probably is responsible for more failures with sweet clover than any other one cause. When sweet-clover plants are not inoculated they must depend upon the available nitrogen in the soil for their supply, and as the crop is grown for the most part on soils low in nitrogen the plants can not be expected to make more than a small growth. ([Fig. 9.])
Arny and Thatcher, at the Minnesota Agricultural Experiment Station, obtained 10 times as much dry matter in the tops and seven times as much in the roots of sweet-clover plants which had been grown on thoroughly inoculated soil as from plants Which had been grown on soil not inoculated. Moreover, the plants grown on the inoculated soil contained 117 pounds more nitrogen to the acre than those grown on the uninoculated soil.
Experiments in many other sections of the country, and especially in the northeastern quarter of the United States, where but little sweet clover or alfalfa has thus far been grown, show that inoculation is very essential to success. Ordinarily it is not necessary to inoculate sweet clover when it is to be planted on land where alfalfa, bur-clover, or black medic thrives, because the same strain of inoculating germs inoculates all of these plants. However, when this closer is to be planted on land where none of the plants inoculated by this strain of the organism have been grown, inoculation should be provided. In localities where sweet clover or other plants inoculated by the same strain of bacteria thrive, the early growth has at times been made much more vigorous by inoculating the soil or seed thoroughly.
It is not safe to assume that a certain piece of soil is inoculated because any one of the plants inoculated by the same strain of the organism is growing or has been grown on other fields in the same vicinity. Many fields have come under observation where sweet clover was a failure because the plants were not inoculated, when plats or fields of alfalfa growing near by were abundantly inoculated.
There are several methods of inoculating sweet clover, any one of which when properly applied should give good results.
The soil-transfer method of inoculation consists in scattering over the field to be seeded 200 to 400 pounds to the acre of soil collected from sweet-clover, alfalfa, or bur-clover fields where the healthy plants show an abundance of tubercles on the roots. It is strongly recommended that this soil be scattered on a cloudy day or in the early morning or in the evening and immediately harrowed or disked into the ground, as the sun's rays are very injurious to the inoculating germs. It is a good plan for the person scattering the soil to walk directly in front of the harrow. When this practice is followed little harm can be done by the light. To facilitate even scattering, the soil may be mixed thoroughly with two or three times its weight of other soil, preferably from the field where the sweet clover is to be sown. Soil used for inoculating sweet clover does not necessarily have to be scattered on the land just previous to sowing the seed. It may be scattered a few months or a year in advance of the time the sweet clover is to be seeded and be just as effective as if it were scattered at a later time. In general, where sweet clover is to be seeded in the spring on winter grain, the inoculation should be applied before the grain is sown.
Good success has been obtained by drying in a dark place soil containing the inoculating germs, sifting it, and running it through the fertilizer compartment of a grain drill. When this method is employed it is not necessary to use as much soil as when it is scattered broadcast.
A comparatively new method which has given successful results calls for dampening each bushel of seed and spreading it on a cloth, paper, or cement floor, where half a gallon of throughly inoculated soil from sweet-clover or alfalfa plants may be sifted over it. Some people prefer to add a trace of glue or sugar to water, so that more of the soil will adhere to the seed, although some soil will remain on the seed if the glue or sugar is not used. When only this quantity of soil is used it should be collected from around the roots of sweet-clover plants which are abundantly inoculated. Such soil may be collected in the fall and kept until spring in a cool, dry, dark place with no injury to the inoculating organisms. Seed treated in this manner should be kept in the dark and should be sown as soon as possible after treating.