Tree-frogs turn green as the result of the contact with leaves. Dark frogs will turn green when put into an absolutely dark vessel in which there are leaves. This is reflex action, and blinded specimens do the same. The principal centres of the nerves which control the chromatophores, lie in the corpora bigemina and in the optic thalami of the brain. When these centres are destroyed, the frog no longer changes colour when put upon leaves, but if a nerve, for instance the sciatic, be stimulated, the corresponding portion of the body, in this case the leg, turns green. Rough surfaces cause a sensation which makes the frog turn dark. Rana seems to depend chiefly upon temperature and the amount of moisture in the air, so far as its changes of colour are concerned. Biedermann concludes that the "chromatic function of frogs in general depends chiefly upon the sensory impressions received by the skin, while that of fishes depends upon the eye."
All this sounds very well, but the observations and experiments are such as are usual in physiological laboratories, and the frogs, when observed in their native haunts, or even when kept under proper conditions, do not always behave as the physiologist thinks they should. There is no doubt that in many cases the changes of colour are not voluntary, but reflex actions. It is quite conceivable that the sensation of sitting on a rough surface starts a whole train of processes: roughness means bark, bark is brown, change into brown; but one and the same tree-frog does not always assume the colour of the bark when it rests, or even sleeps upon, such a piece. He will, if it suits him, remain grass-green upon a yellow stone, or on a white window-frame. I purposely describe such conditions, changes, coincidences, and discrepancies in various species, notably in Hyla arborea, H. coerulea, Rana temporaria, Bufo viridis, to show that in many cases the creature knows what it is about, and that the eye plays a very important part in the decision of what colour is to be produced. The sensory impression received through the skin of the belly is the same, no matter if the board be painted white, black, or green, and how does it then come to pass that the frog adjusts its colour to a nicety to the general hue or tone of its surroundings?
Boulenger[[16]] has given us a summary of the action of the poison of Amphibia:
It is well known to all who have handled freshly-caught newts, and certain toads, especially Bombinator, that their secretion acts as a sternutatory, and causes irritation of the nose and eyes, the effects produced on us by Bombinator being comparable to the early stages of a cold in the head. Many collectors of Batrachians have learned, to their discomfiture, how the introduction of examples of certain species into the bag containing the sport of their excursion may cause the death of the other prisoners; for although the poison has no effect on the skin of individuals of the same species, different species, however closely allied, may poison each other by mere contact. But when inoculated the poison acts even on the same individual.
Miss Ormerod, to personally test the effect, pressed part of the back and tail of a live Crested Newt between the teeth. "The first effect was a bitter astringent feeling in the mouth, with irritation of the upper part of the throat, numbing of the teeth more immediately holding the animal, and in about a minute from the first touch of the newt a strong flow of saliva. This was accompanied by much foam and violent spasmodic action, approaching convulsions, but entirely confined to the mouth itself. The experiment was immediately followed by headache lasting for some hours, general discomfort of the system, and half an hour after by slight shivering fits."
Numerous experiments have shown that the poison of toads, salamanders, and newts is capable, when injected, of killing mammals, birds, reptiles, and even fishes, provided, of course, that the dose be proportionate to the size of the animal. Small birds and lizards succumb as a rule in a few minutes; guinea-pigs, rabbits, and dogs in less than an hour.
This poison of Amphibia is not septic, but acts upon the heart and the central nervous system. That of the common toad has been compared, in its effects, to that of Digitalis and Erythrophlaeum. Some authorities hold that the poison is an acid, others regard it as an alkaloid.
Phisalix[[17]] has come to the conclusion that toads and salamanders are possessed of two kinds of glands, different both anatomically and physiologically. These are, first the mucous glands, spread over the greater part of the body, with an alkaloid secretion, which acts as a narcotic; secondly, specific glands, as the parotoids and larger dorsal glands, the secretion of which is acid, and acts as a convulsive.
The Indians of Colombia are said to employ the secretion of Dendrobates tinctorius for poisoning their arrows. The poison is obtained by exposing the frog to a fire, and after being scraped off the back is sufficient for poisoning fifty arrows. It acts on the central nervous system, and is used especially for shooting monkeys. Concerning the use of this poison for "dyeing" parrots, see p. [272].
The milky secretion of toads protects them against many enemies, although not always against the grass-snake. A dog which has once been induced to bite a toad, suffers so severely that it will not easily repeat the experiment. The handling of tree-frogs also irritates both nose and eyes. The hind limbs of the Water-frog, Rana esculenta, have a very bitter, acrid taste. In short, most, if not all, Amphibia are more or less poisonous, and it is significant that many of the most poisonous, e.g. Salamandra maculosa, Bombinator, Dendrobates, exhibit that very conspicuous combination of yellow or orange upon a dark ground, which is so widespread a sign of poison. Other instances of such warning colours, protective in a defensive sense, are the Wasps and Heloderma, the only poisonous lizard.