(5) Internal body, viscus, or reserve material: Best shown by vital staining with neutral red. This is a granular strand-like body extending from 52·7 per cent. to 65 per cent. of length from head.
(6) Tail end: (i) Rod-like structures resembling those in the head, 90 per cent. of length. (ii) The column of nuclei extends to 95 per cent. of length, so that the terminal portion is free from nuclei.
(7) Mouth: Terminal according to some authors, lateral according to others. Some describe a fang on the head, others not. By vital staining and eosin differentiation two rod-like structures with mushroom-like caps can be seen behind the head.
(8) Cuticle: Transversely striated. There is a longitudinal break in the striation on each side corresponding to the lateral lines. The striation is best shown by vital staining with azur II and eosin differentiation.
(9) Column of nuclei: These nuclei of the gut cells form the main feature in ordinary dry films stained with hæmatoxylin. They are separated by a space from the subcuticular cells.
Fig. 282.—Mf. bancrofti in thick film, dried and stained with hæmatoxylin: 1, shrunken; 2, unshrunken. × 1,000. (After Fülleborn)