Staining.—As under flukes. It is necessary to sacrifice portions of the tapeworm for this purpose, cutting out, e.g., mature segments, so as to study the topography of the genitalia.
Clearing.—As under flukes. To examine the hooks satisfactorily it is best to cut off the head with a sharp knife and mount. A certain amount of pressure is then advisable in order to view the hooks completely so as to measure them.
Preservation of Ova in Fæces, etc.
As under flukes.
Preservation and Examination of Nematodes.
Fixation.—(1) Thoroughly wash the worms to get rid of mucus, etc., by shaking up in warm saline (or water) till the washings are clean. Then transfer to 70 per cent. alcohol heated to about 70° C. It is absolutely necessary to use hot fixatives in order to extend the worms. If no alcohol or spirit is immediately available, drop the worms into hot water, or saline, and transfer later to 70 per cent. alcohol.
(2) Drop into hot 10 per cent. formalin.
Clearing.—(1) Carbolic acid, vide p. [471].
(2) Creasote, vide p. [471].