Charter of 1609.—The lack of success in the original venture had caused those interested to make an effort to enlarge the company. The incorporators of the charter of 1609 were fifty-six of the guilds and companies of London, and six hundred and fifty-nine persons, among whom were included twenty-one peers, ninety-six knights, eleven professional men, and fifty-three captains. The new company was to have the land two hundred miles north and two hundred miles south of Point Comfort and stretching from sea to sea west and northwest, and the islands within one hundred miles of the coast. The government was vested in a council, which was given power to appoint its own officers, to make laws for the government of the colony, and to take in new stockholders. The English church was established as the religion of the colony.

The starving time.—In June, 1609, nine vessels commanded by Newport sailed from England with the new governor, Sir Thomas Gates, and about five hundred emigrants. Beset by pestilence and storms, many died on the voyage, about four hundred being landed at Jamestown in August. The vessel carrying the governor was stranded in the Bermudas, and he did not arrive at Jamestown until May, 1610. There he found the colonists in a frightful condition, dissensions among the officers, Indian attacks, disease, and starvation having brought the colony to the brink of destruction. Gates decided to give up the ill-fated attempt, and taking all the settlers on board, sailed down the James River, but met a vessel bearing the news that a new governor, Lord Delaware, had arrived at Point Comfort with supplies and a hundred and fifty emigrants. Gates immediately returned to Jamestown. Of the nine hundred persons who had been landed in Virginia during the first three years, only one hundred and fifty were alive upon the arrival of Delaware.

Spanish resistance.—Spain regarded the Jamestown colony as an intruder, and both Spaniards and Englishmen considered it as a menace to Spain's northern outposts, and to her merchant fleets, which passed close by on their homeward voyage. Dale remarked that the settlement "wyll put such a byt in our ainchent enemyes mouth as wyll curb his hautynes of monarchie." Zúñiga, Spanish ambassador to England, urged that "such a bad project should be uprooted now, while it can be done so easily."

At Jamestown fear of a Spanish attack was almost constant, and Newport sought aid in England lest the "all devouring Spaniard lay his ravenous hands" upon the infant colony. Spanish resistance had already been felt by way of vigorous diplomatic protest and through the capture of the Richard in 1606. In 1609 a Spanish expedition was sent to Jamestown under Captain Ecija, commander of the garrison at St. Augustine. On July 24 Ecija entered Chesapeake Bay. Concluding that the settlement was too strong to capture with one small vessel, he withdrew, but on his way down the coast he conferred with the Indian tribes, and sent a delegation of natives overland to spy upon the English. On Ecija's return to St. Augustine another native delegation was sent to Virginia from Florida by Governor Ybarra. The success of these embassies has been inferred from the Indian massacres at Jamestown in the following winter. Two years later another Spanish expedition was sent to Jamestown. Captures were made on both sides and the episode was followed by a demand at the English settlement for reinforcements.

Zúñiga continued to urge the destruction of the colony, but Philip III temporized, allured by the hope of an English alliance and encouraged by his informants to believe that the struggling colony would fail through misery. Instead of dying out, however, as time went on Virginia waxed stronger, and soon became a base for attacks on Spanish commerce, as had been predicted. The founding of Jamestown in Axacan was the first English nibble at the Spanish mainland possessions, a process which continued for a century and a half.

Delaware's governorship.—Delaware remained in Virginia less than a year, during which period new colonists arrived, the settlement at Jamestown was rebuilt, the Indians were driven from Kecoughtan, and forts Charles and Henry were established at the mouth of the James River. An expedition was also sent to the falls in search of a gold mine, but it found none. Delaware was unable to check the ravages of disease, and during the summer a hundred and fifty died. The governor left the colony in March, 1611, but remained in office until his death in 1618, during which period the government was administered by deputy governors.

Dale's strong hand.—Sir Thomas Dale was left in charge. He was a brusque old soldier who had seen service in the Netherlands, and during his administration, the colony was governed by military law. The men were forced to work like slaves, and those who rebelled were punished with the greatest severity, several captured runaways being burned at the stake. The Indians along the James and York rivers were attacked; Pocahontas, the daughter of Powhatan, was captured, and the hostage was used to force that powerful chief to make peace. Hearing of the French occupation of Acadia, Dale sent Captain Argall to destroy the settlements.

Charter of 1612.—During the administration of Dale, a change was made in the charter, the powers of the council being considerably enlarged. The Bermudas were also placed under its jurisdiction. The rights in the islands, however, were subsequently sold to some of the members of the London Company, who obtained a charter in 1614 under the name of the Somers Island Company.

Change in the management of the company.—Sir Thomas Smythe had been the moving spirit of the company, but in spite of his efforts, the colony had proved a financial failure, and he was willing to let others carry on the enterprise. The central figure in the company after 1618 was Sir Edwin Sandys. Smythe had realized that it was necessary to change the communal form of ownership to one of landed proprietorship, and had issued instructions that fifty acres of land be assigned to every person who would transport one person to the colony. This policy was carried out by Sandys, and the "old colonists" were allowed to obtain larger tracts of land.

House of Burgesses.—In April, 1619, Sir George Yeardley assumed control as governor of Virginia. He brought out instructions by which the inhabitants of each place and plantation were to elect two burgesses, who were to meet at Jamestown in a general assembly. This first representative assembly in America met in the church at Jamestown on July 30. It was composed of the governor, councilors, and twenty-two burgesses. At the first session, the assembly sat in the two-fold capacity of law makers and court of law.