Common objects of the microscope

by J. G. Wood

In the centre of the mouths may be seen a dark spot, which is the aperture through which the air communicates with the passages between the cells in the interior of the structure. In the flowering plants their shape is generally rounded, though they sometimes take a squared form, and they regularly occur at the meeting of several surface cells. The two kidney-shaped cells which form the “mouth” are the “guard-cells,” so called from their function, since, by their change of form, they cause the mouth to open or shut, according to the needs of the plant. In young plants these guard-cells are very little below the surface of the leaf or skin, but in others they are sunk quite beneath the layer of cells forming the outer coat of the tissue. There are other cases where they are slightly elevated above the surface.

Stomata are found chiefly in the green portions of plants, and are most plentiful on the under side of leaves. It is, however, worthy of notice, that when an aquatic leaf floats on the water, the mouths are only to be found on the upper surface. These curious and interesting objects are to be seen in many structures where we should hardly think of looking for them; for instance, they may be found existing on the delicate skin which envelops the kernel of the common walnut. As might be expected, their dimensions vary with the character of the leaf on which they exist, being large upon the soft and pulpy leaves, and smaller upon those of a hard and leathery consistence. The reader will find ample amusement, and will gain great practical knowledge of the subject, by taking a plant, say a tuft of groundsel, and stripping off portions of the external skin or “epidermis” from the leaf or stem, etc., so as to note the different sizes and shapes of the stomata.

On the opposite bottom corner of Plate I. Fig. [25], is an example of a stoma taken from the outer skin of a gourd, and here given for the purpose of showing the curious manner in which the cells are arranged about the mouth, no less than seven cells being placed round the single mouth, and the others arranged in a partially circular form around them.

Turning to Plate II., we find several other examples of stomata, the first of which (Fig. [1]) is obtained from the under surface of the buttercup leaf, by stripping off the external skin, or “epidermis,” as it is scientifically termed. The reader will here notice the slightly waved outlines of the cell-walls, together with the abundant spots of chlorophyll with which the leaf is coloured. In this example the stomata appear open. Their closure or expansion depends chiefly on the state of the weather; and, as a general rule, they are open by day and closed at night.

A remarkably pretty example of stomata and elongated cells is to be obtained from the leaf of the common iris, and may be prepared for the microscope by simply tearing off a strip of the epidermis from the under side of the leaf, laying it on a slide, putting a little water on it, and covering it with a piece of thin glass. (See Plate II. Fig. [2].) There are a number of longitudinal bands running along the leaf where these cells and stomata appear. The latter are not placed at regular intervals, for it often happens that the whole field of the microscope will be filled with cells without a single stoma, whilst elsewhere a group of three or four may be seen clustered closely together.

Fig. [3] on the same Plate exhibits a specimen of the beautifully waved cells, without mouths, which are found on the upper surface of the ivy leaf. These are difficult to arrange from the fresh leaf, but are easily shown by steeping the leaf in water for some time, and then tearing away the cuticle. The same process may be adopted with many leaves and cuticles, and in some cases the immersion must be continued for many days, and the process of decomposition aided by a very little nitric acid in the water, or by boiling.

On the same Plate are three examples of spiral and ringed vessels, types of an endless variety of these beautiful and interesting structures. Fig. [4] is a specimen of a spiral vessel taken from the lily, and is a beautiful example of a double spire. The deposit which forms this spiral is very strong, and it is to the vast number of these vessels that the stalk owes its well-known elasticity. In many cases the spiral vessels are sufficiently strong to be visible to the naked eye, and to bear uncoiling. For example, if a leaf-stalk of geranium be broken across, and the two fragments gently drawn asunder, a great number of threads, drawn from the spiral vessels, will be seen connecting the broken ends. In this case the delicate membranous walls of the vessel are torn apart, and the stronger fibre which is coiled spirally within it unrolls itself in proportion to the force employed. In many cases these fibres are so strong that they will sustain the weight of an inch or so of the stalk.

In Fig. [5] is seen a still more bold and complex form of this curious structure; being a coil of five threads, laid closely against each other, and forming, while remaining in their natural position, an almost continuous tube. This specimen is taken from the root of the water lily, and requires some little care to exhibit its structure properly.

Every student of nature must be greatly struck with the analogies between different portions of the visible creation. These spiral structures which we have just examined are almost identical in appearance, and to some extent in their function, with the threads that are coiled within the breathing tubes of insects. This is in both cases twofold, namely, to give support and elasticity to a delicate membrane, and to preserve the tube in its proper form, despite the bending to which it may be subjected. When we come to the anatomy of the insect in a future page we shall see this structure further exemplified.

In some cases the deposit, instead of forming a spiral coil, is arranged in a series of rings, and the vessel is then termed “annulated.” A very good example of this formation is given in Fig. [6], which is a sketch of such a vessel, taken from a stalk of the common rhubarb. To see these ringed vessels properly, the simplest plan is to boil the rhubarb until it is quite soft, then to break down the pulpy mass until it is flattened, to take some of the most promising portions with the forceps, lay them on the slide and press them down with a thin glass cover. They will not be found scattered at random through the fibres, which elsewhere present only a congeries of elongated cells, but are seen grouped together in bundles, and with a little trouble may be well isolated, and the pulpy mass worked away so as to show them in their full beauty. As may be seen in the illustration, the number of the rings and their arrangement is extremely variable. A better, but somewhat more troublesome, plan is to cut longitudinal sections of the stem, as described in our concluding chapter, when not only the various forms of cells and vessels, but their relations to each other, will be well shown. The numerous crystals of oxalate of lime, which make rhubarb so injurious a food for certain persons, will also be well seen. These crystals are called “raphides,” and are to be found in very many plants in different forms.