Something like finality appears to have been reached, at least for the present, in the development of the microscope; and whilst it is beyond the scope of this work to treat of the refined and costly apparatus which is essential to useful work in certain departments of research, the result has, on the whole, been highly favourable to the worker of moderate means and ambitions, since lenses are now accessible, at the cost of a few shillings, comparatively speaking, which could not have been purchased at all when this work first appeared. It is with such appliances that we have here to deal. The worker whose finances are restricted must be contented to extemporise for himself many pieces of apparatus, and will find pleasure and occupation in doing so. And let him remember, for his encouragement, that many such home-made appliances will fulfil their purpose quite as well as the imposing paraphernalia of glittering brass and glass which decorates the table of the wealthy amateur. It is not the man who possesses the best or most costly apparatus, but the one who best understands the use of that which he possesses, that will make the most successful microscopist. A good observer will discover, with only the aid of a pocket-magnifier, secrets of Nature which have escaped the notice of a whole army of dilettante microscopists, in spite of the advantages which, as regards instruments, the latter may enjoy.
It is for those who desire to be of the former class that this book is written, and in the course of the following pages instances will be given in which the exercise of a small amount of ingenuity and the expenditure of a few pence will be found equivalent to the purchase of costly and complicated apparatus.
An enormous amount of valuable work was done in the earliest days of microscopy, when the best apparatus available was a single lens, composed of the bead formed by fusing the drawn-out end of a rod of glass. Inserted into a plate of metal, or a piece of card, such a primitive instrument was capable of affording a large amount of information. Similar instruments are to be purchased for a few pence at the present day, and are not without their use for purposes of immediate examination of material. A very common form is a glass marble, ground flat on one side, and mounted in a tube. The material to be examined is placed upon the flat side, and is seen magnified to an extent inversely proportional to the diameter of the sphere of glass.
CHAPTER II
Elementary Principles of Optics—Simple Microscopes—Compound Microscope—Accessory Apparatus—Cover-glasses—Troughs—Condensers—Dissection—Dipping-tubes—Drawing—Measurement.
Before proceeding to deal with the microscope itself, it may be useful to give a short summary of the optical laws upon which its working depends.
To go into the minutiæ of the matter here would be out of place, but it will be found very helpful, especially in the matter of illumination, to acquire some knowledge of, and facility in applying, these elementary principles. We shall confine our remarks to convex lenses, as being the form to which all the combinations in the microscope may be ultimately reduced.
Every convex lens has one “principal” focus, and an infinite number of “conjugate” foci. The principal focus is the distance at which it brings together in one point the rays which fall upon it parallel to its axis, as shown in Fig. [1], in which A is the axis of the lens L, and the rays RR are brought together in the principal focus P. Thus a ready means of finding the focal length of any lens is to see at what distance it forms an image of the sun, or of any other distant object, upon a screen, such as a piece of smooth white cardboard. In the figure this distance will be PL. Conversely, if the source of light be at P, a parallel beam of light will be emitted from the lens.