CHAPTER XI
Hints on the Preparation of Objects—Preservative Fluids—Mounting Media—Treatment of Special Objects.
The microscopist who relies altogether on the dealer for his permanent preparations may expend a good deal of money, but the satisfaction which he derives from his hobby will be very inferior to that experienced by the worker who endeavours to secure, for exhibition or for reference, specimens of the objects which he finds most interesting and instructive to himself.
It will be our endeavour in the following pages to give a summary of the elementary principles upon which reliance is to be placed, though it must be clearly understood that the technique of the subject, already occupying a vast amount of literature, is extending day by day, so that it is impossible to deal exhaustively even with one single section of it. Reference must be made, for further information, to such publications as the Journal of the Royal Microscopical Society, or that of the Quekett Club, or to the monographs on the various departments. Davies’ work on the general subject will also be found useful by the beginner.
Taking first the question of reagents, we may mention five which leave the cells of a tissue as nearly as possible in the natural condition, but fit for permanent preservation. The first of these, in order of importance and of general applicability, is alcohol, represented for most purposes by methylated spirit, which contains about 84 per cent. of absolute alcohol, though, unfortunately for our purpose, there is a certain quantity of mineral naphtha in it in addition. This last has the effect of making it go milky upon dilution with water, which is a considerable disadvantage, though the milkiness disappears to some extent on standing, and it is rarely worth the while of the ordinary microscopist to go through the formalities necessary to obtain permission to purchase unmineralised spirit, which cannot be had in quantities of less than five gallons (as it is only to be had from the distillers under an Excise permit), and distillers may not supply less.
Four parts of methylated spirit with one of water forms the classical “70 per cent.” alcohol, the most generally useful of all fluids for hardening and preserving purposes. A considerable quantity of this fluid should always be available.
Whatever other fluid may be used to begin with, spirit must almost always be used to finish the process, and fit the tissue for section-cutting and staining.
Of purely preservative, or fixative, fluids, we may mention “formalin,” a 40 per cent. solution of formic aldehyde, which is rapidly coming to the front, as indeed it deserves to do. It is but slightly poisonous, if at all, and leaves in the tissue nothing which requires subsequent removal before proceeding to harden for section-work, whilst it is an admirable preservative of cell-form.
Another admirable but highly poisonous reagent is corrosive sublimate, in saturated solution, with 2 per cent. of acetic acid.