We cannot go into it here, further than to say that most sections cut from unstained tissue will yield excellent results if stained first with Delafield’s logwood solution (to be obtained at Baker’s) to a very slight extent, and then with a solution of safranin. The sections should be washed with tap-water after the logwood stain, and should be of a pale violet colour. If over-stained, the colour may to a great extent be removed by washing with a very weak solution of hydrochloric acid, about two drops of acid to each ounce of water, and repeated washing in tap-water to remove the acid, and restore the violet. The safranin stain should be weak, and should be allowed to act for some time. From this last the sections are transferred to strong spirit, the latter being renewed until the sections cease to give up the red dye; and they may then be mounted as described. The results with most tissues are superb, every detail of the structure being splendidly brought out. Safranin alone is also an admirable stain for many purposes.

Further information must be sought in the book already mentioned. Let us, in closing, warn the beginner of two things which are of general application in practical microscopy. The first is, not to be discouraged by failures. The manipulations are in many cases very delicate, and premiums must be paid to experience for insurance against failure in every one of the processes.

The second is, that the most scrupulous cleanliness will hardly suffice to prevent contamination of preparation by the all-pervasive dust which, invisible to the eye, assumes colossal proportions under the microscope, and the particles of which have an unpleasant habit of collecting on the most interesting or most beautiful portion of the preparation. This can only be guarded against by careful filtration of all fluids, and constant watchfulness.

A preparation properly made is a thing of beauty, and a joy for ever,—or if not for ever, at any rate for many years; and one such will repay an infinitude of pains taken in its production.

INDEX

PAGEPAGE
Air-pump,[174]"   mounting of,[177]
Algæ,[78]
"  marine,[92]Jelly-fish,[158]
Anemones, sea,[159]
Antennæ,[96]Larva of Chironomus,[152]
Ants,[97]Light, arrangement of,[29]
Live-box,[17]
Bacillaria,[87]Logwood solution,[183]
Balancers of Fly,[112]
Bark,[61]Magnification, to measure,[27]
Blights,[89]Mare’s tail,[91]
Blood, circulation of,[129]Marine life,[155]
"  corpuscles of,[128]Microscope, Baker’s,[14]
Bone,[123]"    "   “portable”, [15]
Breathing-tubes,[109]"    primitive,[5]
Bull’s-eye, use of,[32]"    simple,[12], [13]
Mildew,[89]
Camera lucida,[25]Mirror, concave,[29]
Canada Balsam,[170]Mollusca,[161]
Cartilage,[124]Mounting,[168]
Cells, animal,[122]"   dry,[173]
"  circulation in,[40]"   foraminifera,[157]
"  mounting dry in,[173]Mosses,[96]
"  pigment,[121], [165]Muscle,[127]
"  spiral,[46]
"  vegetable,[37]Nails,[124]
Ceramidia,[93]Needles,[22]
Chlorophyll,[40]Nerve,[127]
Compressorium, Beck’s,[18]Net,[133]
Condenser, bull’s-eye,[19]Nucleus,[40]
"   substage,[19]
Confervæ,[84]Objectives,[16]
Conjugation,[82], [84]Objects, drawing of,[24]
Convex lenses,[7]"   photography of,[36]
"     foci of,[7], [8]Oil-cells,[58], [61]
"     image formed by,[10]Oscillatoriæ,[84]
"     virtual image,[11]Osmic acid,[170]
Corallines,[164]
Corrosive sublimate,[170]Parasites,[153]
Cover-glasses,[18]Petals,[69]
Picric acid,[170]
Desmids,[81]Pigment,[121]
Diatoms,[85]Pocket magnifiers,[13]
"   preparation of,[175]Polariscope,[166]
Dipping-tubes,[22]Pollen,[71]
Dissection,[20]Polyzoa,[147]
"   instruments,[21]Pond-hunting,[132]
"   under microscope,[24]Preservatives,[169]
Drawing,[25]
"   squares,[26]Radiolaria,[157]
Rotifers,[147]
Echinoderms,[162]
Entomostraca,[152]Safranin stain,[183]
Epidermis, animal,[122]Sap,[128]
"   vegetable,[68]Scent-glands,[57]
Extemporised apparatus,[5]Sea-weeds,[92]
Section-cutting,[178]
Feathers,[119]Seeds,[75]
Fish, scales of,[118]Skin,[120]
"  parasites of,[153]Spiracles,[102]
Fixation of cell-forms,[171]Sponge, fresh-water,[135]
Focus of mirror,[29]"   spicules,[155]
Foraminifera,[156]Sporangia,[92]
Formalin,[164]Stage-forceps,[116]
Frog-plate,[129]Starch,[63]
"   mounting,[172]
Gills of mussel,[122]Stomata,[49]
Gizzard of insects,[109]Suckers,[108]
Glycerine-gelatine,[172]
"   jelly,[171]Teeth,[125]
Troughs, glass,[18]
Hairs, animal,[116]
"  vegetable,[53]Water-bears,[152]
Heads of Insects,[104]Wings,[110]
Wool,[116]
Illumination, correct,[31], [32]Worms, fresh-water,[14]
"    dark-field,[34]"   marine,[160]
"    for opaque objects, [33]
Imbedding,[180]Yeast,[89]
"   by infiltration,[182]
Infusoria,[135]Zoœa,[161]
Injection,[164]Zoophytes,[157]
Insects,[97]Zygnemaceæ,[85]