TABLE III
Tests with Anti-Duck’s-Egg Serum
| Material tested | Amount of precipitum | Percentage | |
|---|---|---|---|
| Duck’s | egg-albumin | .0384 | 100 |
| Pheasant’s | " | .0328 | 185 |
| Fowl’s | " | .0234 | 161 |
| Silver Pheasant’s | " | .0140 | 136 |
| Blackbird’s | " | .0065 | 115 |
| Crane’s | " | .0051 | 114 |
| Moorhen’s | " | .0046 | 112 |
| Thrush’s | " | .0046 | 112 |
| Emu’s | " | .0018 | 105 |
| Hedge-Sparrow’s | " | trace | 10? |
| Chaffinch’s | " | · | 100 |
| Tortoise serum | trace | 10? | |
| Turtle serum | " | 10? | |
| Alligator serum | · | 100 | |
Frog, Amphiuma, and Dogfish sera, as well as Tortoise and Dogfish egg-albumins, were also tested, with negative results.
TABLE IV
Tests with Anti-Fowl’s-Egg Serum
| Material tested | Amount of precipitum | Percentage | |
|---|---|---|---|
| Fowl’s | egg-albumin (old) | .0159 | 100 |
| Fowl’s | " (fresh) | .0140 | 188 |
| Silver Pheasant’s | " | .0075 | 147 |
| Pheasant’s | " | .0075 | 147 |
| Crane’s | " | .0046 | 129 |
| Blackbird’s | " | .0046 | 129 |
| Duck’s | " | .0037 | 123 |
| Moorhen’s | " | .0028 | 118 |
Thrush, Emu, Greenfinch, and Hedge-sparrow egg-albumins were tested and gave traces of precipita, as also did Tortoise and Turtle sera. The egg-albumins of the Tortoise, Frog, Skate, and two species of Dogfish did not react. Alligator, Frog, Amphiuma, and Dogfish sera also yielded no results.[45]
By improving the quantitative method in various ways, Welsh and Chapman[46] were able to explain why the precipitin reaction with egg-white was not strictly specific but gave also, though quantitatively weaker, results with the egg-white of related birds. They found that by a new method devised by them “it is possible to indicate in an avian egg-white antiserum the presence of a general avian antisubstance (precipitin) together with the specific antisubstance.”