From the data given in the table, two curves have been obtained. One of these shows the relation between the hours of the day and temperature; the other exhibits the relation between the hours of the day and the ex­cit­abil­ity as gauged by the amplitude of response ([Fig. 28]). It will be seen that there is, broadly speaking, a marked resemblance between the two curves, which demonstrate the predominant influence of temperature on diurnal variation of ex­cit­abil­ity.

Fig. 28. The continuous curve shows the relation between the hour of the day and temperature. The dotted curve exhibits relation between the hour of the day and ex­cit­abil­ity.

EFFECT OF PHYSIOLOGICAL INERTIA.

It has been shown ([page 59]) that owing to physio­logic­al inertia, the change of ex­cit­abil­ity, generally speaking, lags behind the inducing cause. This fact finds striking illustration in the lag exhibited by the curve of ex­cit­abil­ity in reference to the temperature curve. The minimum temperature was attained at about 4 A.M., but the ex­cit­abil­ity was not reduced to a minimum till four hours later and again there is a marked fall of temperature after 5 P.M., but the ex­cit­abil­ity did not become depressed till two hours later.

There is again the factor of variation of light, the effect of which is not so great as that of temperature. The periods of maximum of light and temperature are, however, not coincident.

We may now discuss in greater detail the diurnal variation of ex­cit­abil­ity in Mimosa, taking the typical case, the record of which is given in [Fig. 23]. The temperature here is seen to remain almost constant, and at an optimum, from 1 to 5 P.M., the condition of light is also favourable. Hence the ex­cit­abil­ity is found to be constant, and at its maximum between these hours. The temperature begins to fall after 6 P.M., and there is, in addition, the depressing action of gathering darkness. Owing to the time-lag, the fall of ex­cit­abil­ity does not commence immediately at 6 P.M., but an hour afterwards, and continues till the next morning. During this period we have the cumulative effect of twelve hours’ darkness and the increasing depression due to cold, the temperature minimum occurring at 4 A.M. On account of the combined effects of these various factors, and phenomenon of lag, the period of minimum ex­cit­abil­ity is in general reached about 8 A.M. In certain other cases this may occur earlier. After the attainment of this minimum, the ex­cit­abil­ity is gradually and continuously increased, under the action of light and of rising temperature, till the maximum is reached in the afternoon.

EFFECT OF SEASON.

It was said that temperature exerted a predominant influence in inducing variation of ex­cit­abil­ity. We may, therefore, expect that the diurnal period would be modified in a certain way according to the season. In winter the night temperature falls very low; hence the depression of ex­cit­abil­ity is correspondingly great, and results in the complete abolition of ex­cit­abil­ity. The after-effect of intense cold is seen in the condition of inexcitability persisting for a very long period in the morning. In summer the prevailing high temperature modifies the diurnal periodicity in a different manner. When the night is warm, the fall of ex­cit­abil­ity is slight. In the day, on the other hand, the temperature may rise above the optimum, bringing about a depression. In such a case the ex­cit­abil­ity in the earlier part of the evening may actually be greater than in the middle of the day. These modi­fi­ca­tions are shown in a very interesting way in the following record (Fig. 29) taken at the end of April. The temperature of Calcutta at this season often rises above 100°F. or 38°C. Table III also exhibits, in the case of the summer specimen, the relation between the hours of the day, temperature, and ex­cit­abil­ity.