XI.—EFFECT OF TEMPERATURE ON GROWTH

By

Sir J. C. Bose,

Assisted by

Surendra Chunder Dass, M.A.

Accurate determination of the effect of temperature on growth presents many serious difficulties on account of numerous complicating factors. In nature, the upper part of the plant is exposed to the temperature of the air, while the root underground is at a very different temperature. Growth, we shall find, is modified to a certain extent by the ascent of sap. (See [p. 189], Expt. 70.) The activity of this latter process is determined by the temperature to which the roots are subjected. The difficulty may be removed to a certain extent by placing the plant in a thermal chamber, with arrangement for regulating the temperature of the air. The air is a bad conductor of heat, and there is some uncertainty of the interior of the plant attaining the temperature of the surrounding air, unless the plant is long exposed to the definite and constant temperature of the plant chamber. Observation of the effects of different temperatures then becomes a prolonged process, with the possibility of vitiation of results by autonomous variation of growth. Reduction of the period of experiment by rapidly raising the temperature of the chamber introduces fresh difficulties; for a sudden variation of temperature often acts like an excitatory shock. This drawback may to some extent be obviated by ensuring a gradual change of temperature. This is by no means an easy process, for even with care the rise of temperature of the air cannot be made perfectly uniform, and any slight irregularity gives rise to sudden fluctuations in the magnified record of growth. Another difficulty arises from the radiation of heat-rays from the sides of the thermal chamber. These rays, I shall in a different Paper show, induce a retardation of growth. The effect of rise of temperature in ac­cel­er­ation of growth is thus antagonised by the action of thermal radiation. This trouble may be minimised by having the inner surface of the thermal chamber of bright polished metal, since the radiating power of a polished surface is relatively feeble.

The contrivance which I employ for ensuring a gradual rise of temperature, consists of a double-walled cylindrical metallic vessel; the plant is placed in the inner chamber, the walls of which are coated with electrically deposited silver and polished afterwards, and at the bottom of which there is a little water. The space between the inner and outer cylinder is filled with water, in which is immersed a coiled copper pipe. Hot water from a small boiler enters the inlet of the coiled pipe and passes through the outlet at the lower end. The water in the outer cylinder is thus gradually raised by flow of hot water in the coiled pipe. The rate of flow of hot water, on which the rate of rise of temperature depends, is regulated by a stop-cock. The air of the inner chamber in which the plant is placed, may thus be adjusted for a definite temperature. The small quantity of water in the inner chamber keeps its air in a humid condition, since dry hot air by causing dessication interferes with normal growth.

METHOD OF DISCONTINUOUS OBSERVATION.