(4) Apply Gram's iodin solution for thirty seconds.
(5) Wash in 95 per cent. alcohol until the purple color ceases to come off.
(6) Wash with equal parts of ether and absolute alcohol, or with ether and absolute alcohol successively.
(7) Apply a saturated aqueous solution of eosin for a minute or two.
(8) Rinse off the eosin with Löffler's methylene-blue, then cover with the methylene-blue, and heat until steam rises.
(9) Wash in water.
(10) Rinse quickly with absolute alcohol.
(11) Apply xylol a half minute or longer.
(12) Mount in balsam.
By this method organisms which stain by Gram's method (staphylococci, streptococci, pneumococci, etc.) are purplish-black; organisms which decolorize by Gram's method (bacilli of Friedländer, influenza bacilli, etc.) are blue; capsules are pink; nuclei of all cells are blue; and granules of eosinophilic cells are bright red.