11th. Changes in the iris and pupil may also be noticed in a good light. The surface should be dark in the horse, and of the various lighter shades in the smaller animals, but in all alike clear, smooth and polished, without variation of shade in spots or patches and without bulging or irregularity at intervals. Apart from the congenital absence of pigment in whole or in part, which may be found in certain sound eyes, a total or partial change of the dark iris of the horse to a lighter red, brown or yellow shade implies congestion, inflammation, or exudation. The corpora nigra in the larger quadrupeds should be unbroken, smooth, rounded, projecting masses outside the free border of the upper portion of the iris. It should show a clear, polished surface like the rest of the iris. The pupil should be evenly oval with its long diameter transversely (horse, ruminant), circular (pig, dog, bird), or round with an elliptical outline on contracting and the long diameter vertical (cat). It should contract promptly in light and dilate as quickly in darkness. Place the patient before a window, cover one eye so as to exclude light, then cover the other eye with the hand and quickly withdraw. The pupil should be widely dilated when the hand is withdrawn and should promptly contract, and it should actively widen and narrow alternately until the proper accommodation has been secured. Any failure to show these movements implies a lesion in the brain, optic nerve, or eye which impairs or paralyzes vision, interferes with accommodation or imprisons the iris. In locomotor ataxia the pupil contracts in accommodation to distance, but not in response to light.
12th. Other causes of pupillary immobility include: (a) Permanence of a pupillary membrane, which has remained from the fœtal condition and may be recognized by oblique focal illumination and invariability of the pupil: (b) Adhesion of the iris to the capsule of the lens—complete or partial—in the latter case the adherent portion only remains fixed, while the remainder expands and contracts, giving rise to distortions and variations from the smoothly curved outline: (c) Adhesion of the iris to the back of the cornea—complete or partial—and leading to similar distortions: (d) Glaucoma in which intraocular pressure determines a permanent dilatation of the pupil and depression of the optic disc: (e) The pupil is narrowed in iritis, and is less responsive to atropia or other mydriatic: (f) Lesions of the oculo-motor nerve may paralyze the iris and fix the pupil. The first three and the fifth of these conditions may be recognized by the naked eye, alone, or with the aid of focal illumination, the fourth may require the aid of the ophthalmoscope and the sixth which cannot be reached by such methods, might in exceptional cases be betrayed by other disorders of the oculo-motor nerve (dropping of the upper eyelid, protrusion of the eyeball, squinting outward).
13th. Coloboma (fenestrated iris), and lacerated iris are recognizable by the naked eye in a good light, or by the aid of focal illumination.
14th. Tension of the eyeball (Tonometry). Elaborate instruments constructed for ascertaining ocular tension are of very little use in the lower animals. The simplest and most practicable method is with the two index fingers placed on the upper lid to press the eyeball downward upon the wall of the orbit using the one finger alternately with the other as if in search of fluctuation. The other fingers rest on the margin of the orbit. All normal eyes have about the same measure of tension and one can use his own eye as a means of comparison. The educated touch is essential. In increased tension, the sense of hardness and resistance, and the indisposition to become indented on pressure is present in the early stages of internal ophthalmias (iritis, choroiditis, retinitis), phlegmon of the eyeball, glaucoma, hydrophthalmos, and tumors of the bulb.
Oblique Focal Illumination.
This is so essential to clear and definite conclusions and is so easily practiced on the domestic animals that every veterinarian should make himself familiar with the method. The method is based on the fact that when two perfectly transparent media touch each other a reflection of luminous rays takes place only at the surface. But in case any opacity exists in any part of the thickness of one of these media, it reflects the rays from its surface no matter what may be its position in the medium. Thus corneal opacities appear as gray blotches and under careful focal illumination it may be determined whether these are on the conjunctival surface, in the superficial or deeper layers of the cornea or in the membrane of Descemet. Similarly cloudiness or floating objects in the aqueous, reflect the luminous rays, and so with opacities in the lens or its capsule, or in the vitreous. In the same way the surface of the iris and corpora nigra may be carefully scrutinized. For satisfactory examination of the media, back of the iris, the pupil should be first dilated, by instillation under the lid of a drop or two of a 3 per cent. solution of atropia, and the examination proceeded with twenty minutes later. Homatropin is preferable to atropin as being less persistent in its action, and less liable to produce conjunctivitis. If it fails to produce the requisite dilatation, it may be followed by a drop of a 4 per cent. solution of hydrochloride of cocaine, which will secure a free dilatation, lasting only for one day in place of seven days as with atropin. The cocaine further removes pain and favors the full eversion of the eyelids.
The instruments required for focal illumination are a biconvex lens of 15 to 20 diopters, and a good oil lamp or movable gas jet. The light of the sun is not satisfactory. The examination ought to be conducted in a dark room, or less satisfactorily in semi-darkness. The lamp is held by an assistant at the level of the eye to be examined, either in front or behind, or first one and then the other, so that the rays of light may fall upon the eye obliquely. If the lids are kept closed it may be necessary to expose the cornea by pressing on the lids with the finger and thumb. The light is held 8 or 10 inches from the eye and the lens is interposed between it and the eye and moved nearer and more distant until the clearest illumination has been obtained of the point to be examined. In this way every accessible part of the eye may be examined in turn. The examiner may make his results more satisfactory by observing the illuminated surface through a lens magnifying three or four diameters. It is important to observe that the eye of the operator must be in the direct line of reflection of the pencil of light.
Cornea. By focusing the light in succession over the different parts of the surface of the cornea, all inflammations, vascularities, opacities, ulcers, and cicatrices will be shown and their outlines clearly defined. By illuminating the deeper layers of the cornea proper, the lesions of keratitis, opacities, ulcers and cicatrices will be shown. To complete the examination of the cornea the light should be focused upon the iris so that it may be reflected back through the cornea. This will reveal the most minute blood-vessels, any cell concretions on Descemet’s membrane, or any foreign body in the cornea which may have been overlooked.
Aqueous Humor. Unless the cornea is densely opaque, the anterior chamber can be satisfactorily explored by the oblique focal illumination. The cloudiness or milkiness of iritis or choroiditis furnishes a strong reflection from its free particles of floating matter, its blood and pus globules, and its flocculi of fibrine. The latter have usually a whitish reflection, the blood elements a red (hypohæma), and the pus a yellow (hypopion). The writhing movements of a filaria scarcely need this mode of diagnosis. Sometimes, and especially in the horse, detached flocculi of black pigment are found floating free in the aqueous and are highly characteristic.
By this illumination one can easily determine the distance of the cornea from the iris and lens (depth of anterior chamber) which is lessened by the forward displacement of iris and lens in undue tension in the vitreous (glaucoma, retinitis, tumors, bladderworms), or of the iris alone, in irido-choroiditis with accumulation of exudate in the posterior chamber of the aqueous. The depth of the anterior chamber may increase in cases of luxation or absence of the lens or softening and atrophy of the vitreous.