2. Before injecting have the subjects arranged in order and record them by name or other means of identification, with age, sex, breed, weight, pregnant or not, past or prospective date of calving, abortion, indications of disease, temperature taken just before injection and appropriate dose.
3. Inject into the loose connective tissue on the side of the neck, the animal being held by the nose, if necessary, by an assistant.
4. Use a syringe which has not been employed for any infectious products, and see that it is thoroughly cleansed and disinfected by boiling or by filling it with a carbolic acid solution (5:100).
5. After drawing the appropriate dose into the syringe, wipe the nozzle and dip it in strong carbolic acid before inserting it into the skin. This safely disinfects any virulent matter that may be lodged on the surface of the skin, and obviates those infected swellings and abscesses that have been a cause of complaint by stock owners.
6. When the nozzle is withdrawn from the skin, wipe it and dip it again into the strong carbolic acid to prevent any risk of infecting the tuberculin into which it is to be plunged.
7. The nozzle is much more easily inserted in the skin if the latter is pinched up so that the needle will transfix it at a right angle, instead of passing through a greater amount of the dense tissue because of the oblique direction. An excited animal with a thick, tense hide and a contracted panniculus muscle will offer serious obstruction which lessens greatly as the subject gets over its excitement and the muscle relaxes.
8. Temperature should be taken at 6 or 7 A. M., eight hours after injection of tuberculin, and every two hours thereafter, until the sixteenth hour.
9. If any subject shows no rise of temperature until the 16th hour after injection, its examination may be discontinued, but if it shows a slight rise toward the 16th hour it should be continued until it has shown a distinct reaction with steady rise and fall, or until, without such distinct reaction, the temperature descends to the normal.
10. If one has shown a distinct reaction but is still rising at the 16th hour, it should be continued till it begins to fall. The typical reaction is one in which the rise and fall are both gradual, and extend over a number of hours.
11. In recording the temperatures, there should be noted the exact time of each feeding, watering and milking, or any other condition (change of weather), which may in any way affect the heat production or radiation.