2. The animal with piroplasma in its blood, did not convey the disease, in the absence of ticks, to a susceptible animal kept with it.
3. The animal with piroplasma in its blood and covered with ticks conveyed the disease to a susceptible animal kept with it.
4. The ticks hatched and raised in glass vessels in the laboratory, when put on susceptible animals, infected them.
5. Ticks taken from cattle harboring the piroplasma, and put on the skin of susceptible animals, or on their pastures in the warm season, infected the exposed stock.
6. The six-legged larvæ developed in the laboratory from the eggs of mature ticks, taken from cattle having the piroplasma, conveyed the disease.
7. On bare pastures as far south as Washington the winter frosts destroyed the ticks so as to render the pastures safe on the following season.
8. Ticks artificially raised in a warm laboratory, produced the disease when placed on susceptible cattle in a warmed stable (65° to 80° F.) in winter.
9. In the Gulf states, in stables which the cattle occupy constantly or enter twice daily for milking or feeding, the ticks may live through the entire winter. The same has occurred in the warm swill stables in the north.
10. When taken into a new locality, it is rarely the mature ovigerous ticks that bite and infect the native cattle of the place, but the next generation of larvæ, so that time must be allowed for the laying and hatching of ova.
11. Ovipositing usually occupies about a week, while hatching varies with the temperature from two to six weeks.