Clinch the experiment thus: Open the door of your chamber and allow the dust to enter it. In three days afterwards you have every vessel within the chamber swarming with bacteria, and in a state of active putrefaction. Here, also, the inference is quite as certain as in the case of the powder sown in your garden. Multiply your proofs by building fifty chambers instead of one, and by employing every imaginable infusion of wild animals and tame; of flesh, fish, fowl, and viscera; of vegetables of the most various kinds. If in all these cases you find the dust infallibly producing its crop of bacteria, while neither the dustless air nor the nutritive infusion, nor both together, are ever able to produce this crop, your conclusion is simply irresistible that the dust of the air contains the germs of the crop which has appeared in your infusions. I repeat there is no inference of experimental science more certain than this one. In the presence of such facts, to use the words of a paper lately published in the 'Philosophical Transactions,' it would be simply monstrous to affirm that these swarming crops of bacteria are spontaneously generated.

Is there then no experimental proof of spontaneous generation? I answer without hesitation, none! But to doubt the experimental proof of a fact, and to deny its possibility, are two different things, though some writers confuse matters by making them synonymous. In fact, this doctrine of spontaneous generation, in one form or another, falls in with the theoretic beliefs of some of the foremost workers of this age; but it is exactly these men who have the penetration to see, and the honesty to expose, the weakness of the evidence adduced in its support.

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And here observe how these discoveries tally with the common practices of life. Heat kills the bacteria, colds numbs them. When my housekeeper has pheasants in charge which she wishes to keep sweet, but which threaten to give way, she partially cooks the birds, kills the infant bacteria, and thus postpones the evil day. By boiling her milk she also extends its period of sweetness. Some weeks ago in the Alps I made a few experiments on the influence of cold upon ants. Though the sun was strong, patches of snow still maintained themselves on the mountain slopes. The ants were found in the warm grass and on the warm rocks adjacent. Transferred to the snow the rapidity of their paralysis was surprising. Ina few seconds a vigorous ant, after a few languid struggles, would wholly lose its power of locomotion and lie practically dead upon the snow. Transferred to the warm rock, it would revive, to be again smitten with death-like numbness when retransferred to the snow. What is true of the ant is specially true of our bacteria. Their active life is suspended by cold, and with it their power of producing or continuing putrefaction. This is the whole philosophy of the preservation of meat by cold. The fishmonger, for example, when he surrounds his very assailable wares by lumps of ice, stays the process of putrefaction by reducing to numbness and inaction the organisms which produce it, and in the absence of which his fish would remain sweet and sound. It is the astonishing activity into which these bacteria are pushed by warmth that renders a single summer's day sometimes so disastrous to the great butchers of London and Glasgow. The bodies of guides lost in the crevasses of Alpine glaciers have come to the surface forty years after their interment, without the flesh showing any sign of putrefaction. But the most astonishing case of this kind is that of the hairy elephant of Siberia which was found incased in ice. It had been buried for ages, but when laid bare its flesh was sweet, and for some time afforded copious nutriment to the wild beasts which fed upon it.

Beer is assailable by all the organisms here referred to, some of which produce acetic, some lactic, and some butyric acid, while yeast is open to attack from the bacteria of putrefaction. In relation to the particular beverage the brewer wishes to produce, these foreign ferments have been properly called ferments of disease. The cells of the true leaven are globules, usually somewhat elongated. The other organisms are more or less rod-like or eel-like in shape, some of them being beaded so as to resemble necklaces. Each of these organisms produces a fermentation and a flavour peculiar to itself. Keep them out of your beer and it remains for ever unaltered. Never without them will your beer contract disease. But their germs are in the air, in the vessels employed in the brewery; even in the yeast used to impregnate the wort. Consciously or unconsciously, he art of the brewer is directed against them. His aim is to paralyze, if he cannot annihilate them.

For beer, moreover, the question of temperature is one of supreme importance; indeed, the recognised influence of temperature is causing on the continent of Europe a complete revolution in the manufacture of beer. When I was a student in Berlin, in 1851, there were certain places specially devoted to the sale of Bavarian beer, which was then making its way into public favour. This beer is prepared by what is called the process of low fermentation; the name being given partly because the yeast of the beer, instead of rising to the top and issuing through the bunghole, falls to the bottom of the cask; but partly, also, because it is produced at a low temperature. The other and older process, called high fermentation, is far more handy, expeditious, and cheap. In high fermentation eight days suffice for the production of the beer; in low fermentation, ten, fifteen, even twenty days are found necessary. Vast quantities of ice, moreover, are consumed in the process of low fermentation. In the single brewery of Dreher, of Vienna, a hundred million pounds of ice are consumed annually in cooling the wort and beer. Notwithstanding these obvious and weighty drawbacks, the low fermentation is rapidly displacing the high upon the Continent. Here are some statistics which show the number of breweries of both kinds existing in Bohemia in 1860, 1865, and 1870 :—

1860. 1865. 1870.
High Fermentation 281 81 18
Low Fermentation 135 459 831

Thus in ten years the number of high-fermentation breweries fell from 281 to 18, while the number of low-fermentation breweries rose from 135 to 831. The sole reason for this vast change — a change which involves a great expenditure of time, labour, and money — is the additional command which it gives the brewer over the fortuitous ferments of disease. These ferments, which, it is to be remembered, are living organisms, have their activity suspended by temperatures below 10°C., and as long as they are reduced to torpor the beer remains untainted either by acidity or putrefaction. The beer of low fermentation is brewed in winter, and kept in cool cellars; the brewer being thus enabled to dispose of it at his leisure, instead of forcing its consumption to avoid the loss involved in its alteration if kept too long. Hops, it may be remarked, act to some extent as an antiseptic to beer. The essential oil of the hop is bactericidal: hence the strong impregnation with hop juice of all beer intended for exportation.

These low organisms, which one might be disposed to regard as the beginnings of life, were we not warned that the microscope, precious and perfect as it is, has no power to show us the real beginnings of life, are by no means purely useless or purely mischievous in the economy of nature. They are only noxious when out of their proper place. They exercise a useful and valuable function as the burners and consumers of dead matter, animal and vegetable, reducing such matter, with a rapidity otherwise unattainable, to innocent carbonic acid and water. Furthermore, they are not all alike, and it is only restricted classes of them that are really dangerous to man. One difference in their habits is worthy of special reference here. Air, or rather the oxygen of the air, which is absolutely necessary to the support of the bacteria of putrefaction, is, according to Pasteur, absolutely deadly to the vibrios which provoke the butyric acid fermentation. This has been illustrated by the following beautiful observation.

A drop of the liquid containing those small organisms is placed upon glass, and on the drop is placed a circle of exceedingly thin glass; for, to magnify them sufficiently, it is necessary that the object-glass of the microscope should come very close to the organisms. Round the edge of the circular plate of glass the liquid is in contact with the air, and incessantly absorbs it, including the oxygen. Here, if the drop be charged with bacteria, we have a zone of very lively ones. But through this living zone, greedy of oxygen and appropriating it, the vivifying gas cannot penetrate to the centre of the film. In the middle, therefore, the bacteria die, while their peripheral colleagues continue active. If a bubble of air chance to be enclosed in the film, round it the bacteria will pirouette and wabble until its oxygen has been absorbed, after which all their motions cease. Precisely the reverse of all this occurs with the vibrios of butyric acid. In their case it is the peripheral organisms that are first killed, the central ones remaining vigorous while ringed by a zone of dead. Pasteur, moreover, filled two vessels with a liquid containing these vibrios; through one vessel be led air, and killed its vibrios in half an hour; through the other he led carbonic acid, and after three hours found the vibrios fully active. It was while observing these differences of deportment fifteen years ago that the thought of life without air, and its bearing upon the theory of fermentation, flashed upon the mind of this admirable investigator.