Redi, as we have seen, proved the maggots of putrefying flesh to be derived from the eggs of flies; Schwann proved putrefaction itself to be the concomitant of far lower forms of life than those dealt with by Redi. Our knowledge here, as elsewhere in connection with this subject, has been vastly extended by Professor Cohn, of Breslau. 'No putrefaction,' he says, 'can occur in a nitrogenous substance if its bacteria be destroyed and new ones prevented from entering it. Putrefaction begins as soon as bacteria, even in the smallest numbers, are admitted either accidentally or purposely. It progresses in direct proportion to the multiplication of the bacteria, it is retarded when they exhibit low vitality, and is stopped by all influences which either hinder their development or kill them. All bactericidal media are therefore antiseptic and disinfecting." [Footnote: In his last excellent memoir Cohn expresses himself thus: Wer noch heut die Faeulniss von einer spontanen Dissociation der Proteinmolecule, oder von einem unorganisirten Ferment ableitet, oder gar aus "Stickstoffsplittern" die Balken zur Stuetze seiner Faeulnisstheorie zu zimmern versucht, hat zuerst den Satz "keine Faeulniss ohne Bacterium Termo" zu widerlegen.'] It was these organisms acting in wound and abscess which so frequently converted our hospitals into charnel-houses, and it is their destruction by the antiseptic system that now renders justifiable operations which no surgeon would have attempted a few years ago. The gain is immense — to the practising surgeon as well as to the patient practised upon. Contrast the anxiety of never feeling sure whether the most brilliant operation might not be rendered nugatory by the access of a few particles of unseen hospital dust, with the comfort derived from the knowledge that all power of mischief on the part of such dust has been surely and certainly annihilated. But the action of living contagia extends beyond the domain of the surgeon. The power of reproduction and indefinite self-multiplication which is characteristic of living things, coupled with the undeviating fact of contagia 'breeding true,' has given strength and consistency to a belief long entertained by penetrating minds, that epidemic diseases generally are the concomitants of parasitic life. 'There begins to be faintly visible to us a vast and destructive laboratory of nature wherein the diseases which are most fatal to animal life, and the changes to which dead organic matter passively liable, appear bound together by what must least be called a very close analogy of causation." [Footnote: Report of the Medical Officer of the Privy Council, 1874, p. 5.] According to this view, which, as I have said, is daily gaining converts, a contagious disease may be defined a conflict between the person smitten by it and a specific organism which multiplies at his expense, appropriating his air and moisture, disintegrating his tissues, or poisoning him by the decompositions incident to its growth.
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During the ten years extending from 1859 to 1869, researches on radiant heat in its relations to the gaseous form of matter occupied my continual attention. When air was experimented on, I had to cleanse it effectually of floating matter, and while doing so I was surprised to notice that, at the ordinary rate of transfer, such matter passed freely through alkalis, acids, alcohols, and ethers. The eye being kept sensitive by darkness, a concentrated beam of light was found to be a most searching test for suspended matter both in water and in air — a test indeed indefinitely more searching and severe than that furnished by the most powerful microscope. With the aid of such a beam I examined air filtered by cotton-wool; air long kept free from agitation, so as to allow the floating matter to subside; calcined air, and air filtered by the deeper cells of the human lungs. In all cases the correspondence between my experiments and those of Schroeder, Pasteur, and Lister in regard to spontaneous generation was perfect. The air which they found inoperative was proved by the luminous beam to be optically pure and therefore germless. Having worked at the subject both by experiment and reflection, on Friday evening, January 21, 1870, I brought it before the members of the Royal Institution. Two or three months subsequently, for sufficient practical reasons, I ventured to direct public attention to the subject in a letter to the Times. Such was my first contact with this important question.
This letter, I believe, gave occasion for the first public utterance of Dr. Bastian in relation to this subject. He did me the honour to inform me, as others had informed Pasteur, that the subject 'pertains to the biologist and physician: He expressed 'amazement' at my reasoning, and warned me that before what I had done could be undone 'much irreparable mischief might be occasioned.' With far less preliminary experience to guide and warn him, the English heterogenist was far bolder than Pouchet in his experiments, and far more adventurous in his conclusions. With organic infusions he obtained the results of his celebrated predecessor, but he did much more — the atoms and molecules of inorganic liquids passing under his manipulation into those more 'complex chemical compounds,' which we dignify by calling them 'living organisms.' [Footnote: 'It is further held that bacteria or allied organisms are prone to be engendered as correlative products, coming into existence in the several fermentations, just as independently as other less complex chemical compounds.' — Bastian, Trans. of Pathological Society, vol. xxvi. 258.]
As regards the public who take an interest in such things, and apparently also as regards a large portion of the medical profession, our clever countryman succeeded in restoring the subject to a state of uncertainty similar to that which followed the publication of Pouchet's volume in 1859.
It is desirable that this uncertainty should be removed from all minds, and doubly desirable on practical grounds that it should be removed from the minds of medical men. In the present article, therefore, I propose discussing this question face to face with some eminent and fair-minded member of the medical profession who, as regards spontaneous generation, entertains views adverse to mine. Such a one it would be easy to name; but it is perhaps better to rest in the impersonal. I shall therefore simply call my proposed co-enquirer my friend. With him at my side, I shall endeavour, to the best of my ability, so to conduct this discussion that he who runs may read and that he who reads may understand.
Let us begin at the beginning. I ask my friend to step into the laboratory of the Royal Institution, where I place before him a basin of thin turnip slices barely covered with distilled water kept a temperature of 120° Fahr. After digesting the turnip for four or five hours we pour off the liquid, boil it, filter it, and obtain an infusion as clear as filtered drinking water. We cool the infusion, test its specific gravity, and find it to be 1006 or higher – water being 1000. A number of small clean empty flasks, of the shape shown on the margin, are before us. One of them is slightly warmed with a spirit-lamp, and its open end is then dipped into the turnip infusion. The warmed glass is afterwards chilled, the air within the flasks cools, contracts, and is followed in its contraction by the infusion. Thus we get a small quantity of liquid into the flask. We now heat this liquid carefully. Steam is produced, which issues from the open neck, carrying the air of the flask along with it. After a few seconds' ebullition, the open neck is again Plunged into the infusion. The steam within the flask condenses, the liquid enters to supply its place, and in this way we fill our little flask to about four-fifths of its volume. This description is typical; we may thus fill a thousand flasks with a thousand different infusions.
I now ask my friend to notice a trough made of sheet copper, with two rows of handy little Bunsen burners underneath it. This trough, or bath, is nearly filled with oil; a piece of thin plank constitutes a kind of lid for the oil-bath. The wood is perforated with circular apertures wide enough to allow our small flask to pass through and plunge itself in the oil, which has been heated, say, to 250° Fahr. Clasped all round by the hot liquid, the infusion in the flask rises to its boiling point, which is not sensibly over 212° Fahr. Steam issues from the open neck of the flask, and the boiling is continued for five minutes. With a pair of small brass tongs, an assistant now seizes the neck near its junction with the flask, and partially lifts the latter out of the oil. The steam does not cease to issue, but its violence is abated. With a second pair of tongs held in one hand, the neck of the flask is seized close to its open end, while with the other hand a Bunsen's flame or an ordinary spirit flame is brought under the middle of the neck. The glass reddens, whitens, softens, and as it is gently drawn out the neck diminishes in diameter, until the canal is completely blocked up. The tongs with the fragment of severed neck being withdrawn, the flask, with its contents diminished by evaporation, is lifted from the oil-bath perfectly sealed hermetically.
Sixty such flasks filled, boiled, and sealed in the manner described, and containing strong infusions of beef, mutton, turnip, and cucumber, are carefully packed in sawdust, and transported to the Alps. Thither, to an elevation of about 7,000 feet above the sea, I invite my co-enquirer to accompany me. It is the month of July, and the weather is favourable to putrefaction. We open our box at the Bel-Alp, and count out fifty-four flasks, with their liquids as clear as filtered drinking water. In six flasks, however, the infusion is found muddy. We closely examine these, and discover that every one of them has had its fragile end broken off in the transit from London. Air has entered the flasks, and the observed muddiness is the result. My colleague knows as well as I do what this means. Examined with a pocket-lens, or even with a microscope of insufficient power, nothing is seen in the muddy liquid; but regarded with a magnifying power of a thousand diameters or so, what an astonishing appearance does it present! Leeuwenhoek estimated the population of a single drop of stagnant water at 500,000,000: probably the population of a drop of our turbid infusion would be this many times multiplied. The field of the microscope is crowded with organisms, some wabbling slowly, others shooting rapidly across the microscopic field. They dart hither and thither like a rain of minute projectiles; they pirouette and spin so quickly round, that the retention of the retinal impression transforms the little living rod into a twirling wheel. And yet the most celebrated naturalists tell us they are vegetables. From the rod-like shape which they so frequently assume, these organisms are called 'bacteria' — a term, be it here remarked, which covers organisms of very diverse kinds.
Has this multitudinous life been spontaneously generated in these six flasks, or is it the progeny of living germinal matter carried into the flasks by the entering air? If the infusions have a self-generative power, how are the sterility and consequent clearness of the fifty-four uninjured flasks to be accounted for? My colleague may urge — and fairly urge — that the assumption of germinal matter is by no means necessary; that the air itself may be the one thing needed to wake up the dormant infusions. We will examine this point immediately. But meanwhile I would remind him that I am working on the exact lines laid down by our most conspicuous heterogenist. He distinctly affirms that the withdrawal of the atmospheric pressure above the infusion favours the production of organisms; and he accounts for their absence in tins of preserved meat, fruit, and vegetables, by the hypothesis that fermentation has begun in such tins, that gases have been generated, the pressure of which has stifled the incipient life and stopped its further development. [Footnote: Beginnings of Life, vol. i. p. 418.] This is the new theory of preserved meats. Had its author pierced a tin of preserved meat, fruit, or vegetable under water with the view of testing its truth, he would have found it erroneous. In well-preserved tins he would have found, not an outrush of gas, but an inrush of water. I have noticed this recently in tins which have lain perfectly good for sixty-three years in the Royal Institution. Modern tins, subjected to the same test, yielded the same result. From time to time, moreover, during the last two years, I have placed glass tubes, containing clear infusions of turnip, hay, beef, and mutton, in iron bottles, and subjected them to air-pressures varying from ten to twenty-seven atmospheres — pressures, it is needless to say, far more than sufficient to tear a preserved meat tin to shreds. After ten days these infusions were taken from their bottles rotten with putrefaction and teeming with life. Thus collapses an hypothesis which had no rational foundation, and which could never have seen the light had the slightest attempt been made to verify it.