To this class belong also the purely digestive puers such as pancreatic juice, intestinal juice and the like, or purified enzymes. These enzymes are colloidal and therefore incapable of diffusion, and consequently they digest the hide mainly from the surface, that is to say, the grain side and inner side of the hide. The fibres, in-so-far as they are accessible at all to the action of the colloidal enzymes, are removed from outside without the fibrils being loosened and straightened. Hence they are weakened by the digestive puer to such an extent that the hides seem to be gradually pulled down; but the skin will by that time have lost too much substance, so that, inter alia, even the grain appears to have been corroded. In this way the leather is rendered thinner and lighter than that from the dung or bacterial puer—a defect manifest to every expert.

This danger is particularly great when, by the artificial admixture of corporeal juices, complications are set up, that intensify the destructive action of the digestive enzymes on the material, and when their supplementary action is not suitably controlled.

These animal or digestive enzymes and fat decomposing substances originate, in-so-far as they are not of vegetable origin, for the most part in the upper portions of the digestive tract. The use of these substances effects an imitation of the purely enzymatic and fat decomposing processes that go on in the upper part of the intestines, but omits the processes—not less important for digestion and the formation of excrement—occurring in the large intestine and the adjoining portions of the digestive tract. Here the bacteria and the vegetable enzymes secreted thereby, play the chief part. They decompose not only the food constituents—partly by putrefaction—but also the albuminoid digestive secretions and the gall, so that only small quantities thereof are left. Hence when applied to the puering process, the use of bacteria, together with their specific enzymes and metabolic products, is equivalent to a restriction of the immoderate digestive action of the pancreatic and digestive juices, i.e. of the animal enzymes.

Digestion therefore, with the resulting formation of excrement, is carried on with the accompaniment of a mutual control, excitation and restriction on the part of the digestive secretions and the bacterial phenomena.

These latter form the real basis of the puering of hides in dung preparations.

This is also evident from the purely practical experience that dog dung puer, e.g. for glove leather, is essentially improved by leaving the dung to ferment for several weeks or rather months. During this process an increase occurs in the micro-organisms present, and also in their metabolic products and enzymes, but not in the digestive juices originating in the organs of the animal body—the term “digestive juices” being restricted to indicate only such juices as are produced in the course of the digestive tract by the organs and cells of the animal body, in contradistinction to the juices produced by bacteria.

According to the present invention, the bacterial cultures, together with their metabolic products or products of digestion and enzymes, are used in combination with one or more of the substances that are excreted in the digestive tract by the animal body itself in order that not only each of these substances may exert its special action on the skin, but also that the aforesaid mutual influencing, and more particularly regulating of the action may take place.

On this principle, in preparing an artificial puer for hides, and in accordance with the nature and preliminary treatment of the raw material, and the requirements put forward in respect of the finished product, the bacterial puer, nutrient medium or culture is mixed with the corresponding digestive juices, or with purified animal or vegetable enzymes, as well as with fat decomposing media, if necessary, such as ground castor-oil seeds, hemp seed or the like, or with extracts of same, or with gall. Where it seems necessary, substances capable of dissolving lime may also be added, for example, sugar, molasses, salts of ammonia, organic acids, or the corresponding salts or the like.

Finally, also, the several operations may be carried out independently of one another, it being in such case again advisable to adhere to the processes going on in the intestines.

If, for instance, specially hard goat skins are to be pulled down strongly by puering, it is advisable to add to the nutrient medium of the bacteria a little papayotin or papain, either—according as rendered necessary by the local conditions—in the preparation of the bacterial puering liquor, or during the propagation of the bacteria. If the fells are less hard so that less pulling down is required, the hides are first treated with a weak solution of the digestive ferments, and then with the bacterial liquor. In this way excessive digestive action is counteracted in good time.