[20] Lambling, Précis de Biochimie, 1911, p. 221.
[21] Another sample contained 136 grm. dry matter.
[22] Gaultier. See also analysis given in Chapter [VI].
[23] Abderhalden, Physiological Chemistry.
[24] Cf. Mann. Chemistry of the Proteids, 1906, p. 432.
[25] The cholesterol was obtained by extracting the dry puer with ether, saponifying with alcoholic potash. Shaking out the unsaponifiables with ether, evaporating to dryness. The residue was taken up with absolute alcohol, decolorized with animal charcoal. On cooling, characteristic crystals of cholesterol separated. Hoppe-Seyler finds from 1 per cent. to 4 per cent. cholesterol in fæces. See also Gaultier, Coprologie Clinique, p. 160.
[26] For qualitative tests for the colouring matters, see Gaultier, Précis de Coprologie Clinique, p. 159.
[27] English Patent, 21202, 1909. See also Chapter [VIII].
[28] Probably syntonin, or bodies of a similar nature (see Allen. Comm. Org. Anal. iv. p. 4). The supposition is supported by the fact that solutions of puer have a slight laevorotatory action on polarized light.
See also note on Van Liers paper, Coll. 376, 1909, p. 823.