The last of the four life requisites we have to consider is that of oxygen. The chick in the shell, like a fish, breathes oxygen which is dissolved in a liquid. A special breathing organ is developed for the chick during its embryonic stages and floats in the white and absorbs the oxygen and gives off carbon dioxide. The amount of this breathing that occurs in the chick is at first insignificant, but increases with development. At no time, however, is it anywhere equal to that of the hatched chicks, for the physiological function to be maintained by the unhatched chicks requires little energy and little oxidation.

Upon the subject of ventilation in general, a great misunderstanding exists. Be it far from me to say anything that will cause either my readers or his chickens to sleep less in the fresh air, yet for the love of truth and for the simplification of the problem of incubation, the real facts about ventilation must be given.

In breathing, oxygen is absorbed and carbon dioxide and water vapor are given off. It is popularly held that abundance of fresh air is necessary to supply the oxygen for breathing and that carbon dioxide is a poison. Both are mistakes. The amount of oxygen normally in the air is about 20 per cent. Of carbon dioxide there is normally three hundredths of one per cent. During breathing these gasses are exchanged in about equal volume. A doubling or tripling of carbon dioxide was formerly thought to be "very dangerous." Now, if the carbon dioxide were increased 100 times, we would have only three per cent., and have seventeen per cent. of oxygen remaining. This oxygen would still be of sufficient pressure to readily pass into the blood. We might breathe a little faster to make up for the lessened oxygen pressure. In fact such a condition of the air would not be unlike the effects of higher altitudes.

Some investigations recently conducted at the U.S. Experiment Station for human nutrition, have shown the utter misconception of the old idea of ventilation. The respiratory calorimeter is an air-tight compartment in which men are confined for a week or more at a time while studies are being made concerning heat and energy yielded by food products. It being inconvenient to analyze such an immense volume of air as would be necessary to keep the room freshened according to conventional ventilation standards, experiments were made to see how vitiated the air could be made without causing ill effects to the subject.

This led to a remarkable series of experiments in which it was repeatedly demonstrated that a man could live and work for a week at a time without experiencing any ill effects whatever in an atmosphere of his own breath containing as high as 1.86 per cent. of carbon dioxide, or, in other words, the air had its impurity increased 62 times. This agrees with what every chemist and physiologist has long known, and that is that carbon dioxide is not poisonous, but is a harmless dilutant just as nitrogen. This does not mean that a man or animal may not die of suffocation, but that these are smothered, as they are drowned, by a real absence of oxygen, not poisoned by a fraction of 1 per cent. of carbon dioxide.

In the same series of experiments, search was made for the mysterious poisons of the breath which many who had learned of the actual harmlessness of carbon dioxide alleged to be the cause of the ill effects attributed to foul air. Without discussion, I will say that the investigators failed to find such poisons, but concluded that the sense of suffocation in an unventilated room is due not to carbon dioxide or other "poisonous" respiratory products, but is wholly due to warmth, water vapor, and the unpleasant odors given off by the body.

The subject of ventilation has always been a bone of contention in incubator discussions. With its little understood real importance, as shown in the previous section, and the greatly exaggerated popular notions of the importance of oxygen and imagined poisonous qualities of carbon dioxide, the confusion in the subject should cause little wonder.

A few years ago some one with an investigating mind decided to see if incubators were properly ventilated, and proceeded to make carbon dioxide determinations of the air under a hen and in an incubator. The air under the hen was found to contain the most of the obnoxious gas. Now, this information was disconcerting, for the hen had always been considered the source of all incubator wisdom. Clearly the perfection of the hen or the conception of pure air must be sacrificed. Chemistry here came to the rescue, and said that carbon dioxide mixed with water, formed an acid and acid would dissolve the lime of an egg shell. Evidently the hen was sacrificing her own health by breathing impure air in order to soften up the shells a little so the chicks could get out. Since it could have been demonstrated in a few hours in any laboratory, that carbon dioxide in the quantities involved, has no perceptible effect upon egg shells, it is with some apology that I mention that quite a deal of good brains has been spent upon the subject by two experiment stations. The data accumulated, of course, fails to prove the theory, but it is interesting as further evidence of the needlessness in the old fear of insufficient ventilation.

At the Ontario Station, the average amounts of carbon dioxide under a large number of hens was .32 of one per cent., or about ten times that of fresh air, or one-sixth of that which the man breathed so happily in the respiratory calorimeter. With incubators, every conceivable scheme was tried to change the amount of carbon dioxide. In some, sour milk was placed which, in fermenting, gives off the gas in question. Others were supplied with buttermilk, presumably to familiarize the chickens with this article so they would recognize it in the fattening rations. In other machines, lamp fumes were run in, and to still others, pure carbon dioxide was supplied. The percentage of the gas present varied in the machines from .06 to .58 of one per cent. The results, of course, vary as any run of hatches would. The detailed discussion of the hatches and their relation to the amount of carbon dioxide as given in Bulletin 160 of the Ontario Station, would be unfortunately confusing to the novice, but would make amusing reading for the old poultryman. Speaking of a comparison of two hatches, the writer, on page 53 of the bulletin says, "The increase in vitality of chicks from the combination of the carbon dioxide and moisture over moisture only, amounting, as it does, to 4.5 per cent. of the eggs set, seems directly due to the higher carbon dioxide content." I cannot refrain from suggesting that if my reader has two incubators, he might set up a Chinese prayer machine in front of one and see if he cannot in like manner demonstrate the efficacy of Heavenly supplications in the hatching of chickens.

The practical bearing of the subject of ventilation in the small incubator is almost wholly one of evaporation. The majority of such machines are probably too much ventilated. In a large and properly constructed hatchery, such as is discussed in the last section of this chapter, the entire composition of the air, as well as its movement, is entirely under control. Nothing has yet been brought to light that indicates any particular attention need be given to the composition of such air save in regard to its moisture content, but as the control of this factor renders it necessary that the air be in a closed circuit, and not open to all out-doors, it will be very easy to subject the air to further changes such as the increasing oxygen, if such can be demonstrated to be desirable.