5. Does the Calisaya prefer the deepest shadows of the forest, does it grow there quite apart from other trees, or is it more frequently found in the open spaces where it is warmed by the sun's rays, such places being usually rather clear of trees? Does it grow solitary, or is it found in groups or clusters, and are its special peculiarities in this respect observable in every forest? Is it observed to be more numerous towards the edge of the forest, and does it evince a tendency to extend thence over the grass, the drift, the plateaux, &c., and what alterations do these make in its habits?
6. Information is wanted as to the month in which the Calisaya blossoms, and that in which the fruit ripens, as also what length of time usually elapses between the first appearance of the buds and the shedding of the corolla, and from the shedding of the corolla to the bursting, i. e. the complete maturity of the capsules. It would seem that in Java it takes a much longer time, as also that it blossoms at an entirely different season from that in which it blossoms in its native regions.
7. Much anxiety is felt as to whether it is possible to ascertain with accuracy how many years old, as also what are the usual height and the diameter (at the base of the trunk) of a Calisaya tree, when it first begins to blossom, and whether these first blossoms are developed into ripe fruit, with seeds capable of fertilization.
8. How high, how thick, and how old are—
a. The youngest and smallest, and
b. The largest and oldest,
Calisaya trees, which are now felled for their bark in South America? What description of bark is the most prized, that from the young and slender, or that from the larger and older trees? Also whether the bark of a very young tree, e. g. four years old, contain thus early the active principle, genuine?
9. As, judging by appearances, it has been rightly assumed that the bark of any given description of Cinchona is found to be more abundantly provided with alkaloid, especially quinine, the greater the elevation above the sea, and becomes impoverished in these respects in proportion as a lower level and a warmer climate are reached, it is desirable that special observations should be made for the elucidation of these particulars.
10. It is desirable information should be got from the China bark collectors (Cascarilleros) of Peru, as to the natural foes of the Cinchona plant, especially C. Calisaya, and it appears likewise important to ascertain whether the Calisaya is there also liable to be injured and bored into by mites and other noxious insects.
11. It is highly desirable that all the above recommended observations made respecting Cinchona Calisaya, may also be applied to all other species of Cinchona that may occur in South America, of which those ranking next in interest and importance to us in Java, and which have been planted here, are C. Condaminea, var. lucumæfolia, laurifolia, lanceolata, as also C. cordifolia, C. ovata, and var. erythroderma.