The Equifex (Defries) contains no steam jacket, but coils of pipes are placed at the top and bottom of the apparatus, with the object of imparting to the steam as much heat as is lost by radiation through the walls of the disinfecting chamber, and at the same time of preventing undue condensation. The air is first removed by a preliminary current of steam, after which steam at a pressure of ten pounds is intermittently introduced and allowed to escape. The object of this proceeding is to remove air from the pores of the articles to be disinfected by the sudden expansion of the film of water previously condensed on their surface. The apparatus introduced by Dr. Thresh was constructed with a view of overcoming the objection to some of the other machines that bulky articles retained a large percentage of moisture, thus necessitating the use of some additional drying apparatus. A central chamber receives the articles to be disinfected, and is surrounded by a boiler containing a solution of calcium chloride at a temperature of 225° F. This is heated by a small furnace, and the steam given off (218–300° F.) is conducted into the central chamber. The steam is not confined under any pressure except that of the atmosphere. When the steam has passed for a sufficient length of time, it is readily diverted into the open air. Hot air is now introduced, and at the expiration of an hour the articles may be taken out disinfected and as dry as they were when inserted. The apparatus is comparatively inexpensive, and not of a complicated nature. The current steam is saturated, and at a temperature a few degrees above the boiling-point. Many experiments have been performed with this apparatus, and there is now a large amount of evidence in favour of it and current steam disinfection.
Reck's apparatus is another kind of saturated steam disinfector, which resembles the Equifex, but differs from it in employing steam as a current.
It is probable that many other forms of steam disinfector will be invented, and each will have its enthusiastic supporters. Even at the time of writing some excellent results are announced from America.
2. The effects of chemical substances as solutions, or in spray form, upon bacteria have been observed from the earliest days of bacteriology. To some decomposing matter or solution a disinfectant was added and sub-cultures made. If bacteria continued to develop, the disinfection had not been efficient; if, on the other hand, the sub-culture remained sterile, disinfection had been complete. From such rough-and-ready methods large deductions were drawn, and it is hardly too much to say that no branch of bacteriology contains such a vast mass of unassimilated and unassimilable statements as that relating to research into disinfectants. Most of the tabulated and recorded results are conspicuous in having no standard as regards bacterial growth. Yet without such a standard results are not comparable.
Silk threads, impregnated with anthrax spores, were placed in bottles containing carbolic acid of various strengths, and at stated periods threads were removed and placed in nutrient media, and development or otherwise observed. But, as Professor Crookshank[103] has pointed out, this method is fallacious, the thread being still wet with the solution when transferred to the medium, and thus modified in culture, possibly even inhibited altogether. It is unnecessary for us here to discuss every mode adopted by investigators in similar researches. We may just mention that the most approved methods at the present time are based upon two simple plans of exposure. In one we use a known volume of recent broth culture of an organism grown under specified conditions. To this is added a measured quantity of the antiseptic. At stated periods loopfuls of the broth and antiseptic mixture are sub-cultured in fresh-sterilised broth, and resulting development or otherwise closely observed. The other method is practicable when we are dealing with volatile bodies. In such cases a standard culture is made of the organism in broth at a standard temperature. Into this are dipped small strips of sterilised linen. When thoroughly impregnated these are removed from the broth and subsequently dried over sulphuric acid in a vacuum at 38° C. These may now be exposed for a longer or shorter period to the fumes of the antiseptic in question, and broth cultures made at the end of the exposure. It is obvious that a very large number of modifications are possible of these two simple devices for testing the bactericidal power of chemical substances. It should be remembered that here, perhaps, more than anywhere else in bacteriological research, careful control experiments are absolutely necessary.
Mineral acids (nitric, hydrochloric, sulphuric), especially concentrated, are all germicides.
The halogens—chlorine, bromine, iodine, and fluorine—are, all four, disinfectants, but not used in practice. They are named in their order of power as such.
A number of separate bodies, such as chloroform and iodoform, have been much advocated as antiseptics. The cost of the former and odour of the latter have, however, greatly militated against their general adoption.
Chloride of lime is a powerful disinfectant. Professor Sheridan Delépine and Dr. Arthur Ransome have demonstrated its germicidal effect as a solution applied directly to the walls of rooms inhabited by tuberculous patients.[104] It may also be used in solid form for dusting decomposing matter.