Change of weight of stimulated leg—
| After 8 hours | +.050. |
| After 16 hours | +.080. |
| After 24 hours | +.101. |
This result shows that muscular work provoked by electric stimulation noticeably increases the osmotic pressure of the muscle.
In order to discover the exact osmotic pressure in the stimulated muscles we repeated the series of experiments, using more and more concentrated solutions. In a solution whose freezing point was -.57°, we obtained the following values:—
Change of weight of untired leg—
| After 8 hours | -.000. |
| After 16 hours | -.004. |
| After 24 hours | -.006. |
Change of weight of stimulated leg—
| After 8 hours | +.039. |
| After 16 hours | +.072. |
| After 24 hours | +.099. |
Finally, in a solution freezing at -.72°, i.e. with an osmotic pressure at 15° C. of 9.176 atmospheres, we obtained the following mean values for the untired leg:—