In the preparation showing artificial cells the cellular structure is not directly visible until the gelatine has dried. One sees only a gelatinous mass analogous to the protoplasm of a living organism. This mass is nevertheless organized, or at least in process of organization, as we may see by the refraction when its image is projected on the screen.
During the cell-formation, and as long as there is any difference of concentration in the gelatine, each cell is the arena of active molecular movement. There is a double current, as in the living cell, a stream of water from the periphery to the centre, and of the solute from the centre to the periphery. This molecular activity—the life of the artificial cell—may be prolonged by appropriate nourishment,
i.e. by continually repairing the loss of concentration at the centre of the cell.
The life of the artificial cell may also be prolonged by maintaining around it an appropriate medium. If we prematurely dry such a preparation of artificial cells, the molecular currents will cease, to recur again when we restore the necessary humidity to the preparation. This to my mind gives us a most vivid picture of the conditions of latent life in seeds and many rotifera.
These artificial cells, like living organisms, have an evolutionary existence. The first stage corresponds to the process of organization, the gelatine representing the blastema, and the drop the nucleus. Thus the cell becomes organized, forming its own cytoplasm and its own enveloping membrane.
The second stage in the life of this artificial cell is the period during which the metabolism of the cell is active and tends to equalize the concentration of the liquid in the cell and in the surrounding medium.
The third stage is the period of decline. The double molecular current gradually slows down as the difference of concentration decreases between the cell contents and its entourage. When this equality of concentration has become complete the molecular currents cease, the cell has terminated its existence; it is dead. The currents of substance and of energy have ceased to flow—the form only remains.
These artificial cells are sensible to most of the influences which affect living organisms. Like living cells they are influenced both in their organization and in their development by humidity, dryness, acidity, or alkalinity. They are also greatly affected by the addition of minute quantities of chemical substances either to the gelatinous blastema or to the drops which represent the primary nuclei. We may in this way obtain endless varieties, nuclei which are opaque or transparent, with or without a nucleolus, and cells containing homogeneous cytoplasm without a nucleus. We may also obtain cells with cytoplasm filling the whole of the cellular cavity or separated from the cell-membrane. We may obtain
cells imitating all the natural tissues, cells without a membranous envelope, cells with thick walls adhering to one another, or cells with wide intracellular spaces.
