A still better system is where the section-knife is held in a frame which slides on the plate so that the edge is kept just above the surface. In this way the edge of the knife is never dulled by grinding on glass or metal. A common mistake in this form, however, is to raise the cutting edge too far above the surface of the plate. This gives rise to great irregularities.
NOTE F., Page 22.
As the size of the sections which it may be desired to cut, varies within wide limits, the best microtomes made in this country are furnished with as many as three tubes or “bushings,” fitting one within the other, and in this way the hole may be partially filled up and thus reduced in size. This enables us to obtain a range of from one inch and a quarter, suitable for large histological preparations, to one-eighth of an inch for such objects as hair sections.
NOTE G., Page 37.
KNIVES FOR CUTTING SECTIONS.
Success in cutting sections depends more upon the excellence of the knife that is used than upon any other one point, and therefore the art of keeping the knife in good order is one which should be acquired by every microscopist, even at the cost of considerable time, study and labor. As a stepping stone to the acquisition of this art, there are certain general principles which must be thoroughly understood before we can hope for success in practice. These principles are simple, and when once clearly stated, quite obvious, but they are frequently overlooked.
Cutting instruments act in two ways—either as simple wedges or as a series of wedges, the latter being generally known as the saw-like action. It is frequently stated in school philosophies that the edges of all cutting instruments are toothed like a saw, and that they act like a saw; this is evidently not the case with many, such as the axe, the chisel, the plane, etc., and it is not even true of several to which it is frequently applied, such as the razor, the penknife, etc. The action can be said to be “saw-like” only when the teeth act as independent wedges and remove a small portion of the substance acted upon, the portion being known as saw-dust. Now in cutting sections of wood with a sharp razor, where but a single stroke is used to carry the cutting edge across a section, say half an inch in diameter, the edge of the razor does not act like a saw; none of the material is removed except the section itself; in other words, there is no saw-dust. That the edge of the knife or razor is not even, is very easily proved by means of the microscope, but it will be found that the notches and projections form a series of lancet-shaped knifelets, each one of which acts just as does the blade of a penknife when drawn over a piece of wood or a quire of paper. Now if we lay a quire of paper on a board and draw the edge of a penknife over it, we will cut down through the paper, not by sawing but by a moving wedge. It is precisely in this way that the little lancet-shaped teeth on the edge of a razor act when the latter is used to cut a section.
If we further examine the edge of the razor, we will find that the bottoms of the notches are comparatively blunt; if therefore we press the razor directly forward we soon press these blunt portions of the general edge against the material to be cut, and the resistance becomes so great that the material is actually crushed or torn. This would not happen if we had an absolutely perfect edge—one without any notches. This being practically unattainable, however, we must endeavor to make up for it by a sawing motion, (though not a sawing action) at the same time seeking to avoid as much as possible those saw-like features which render this motion necessary. We have dwelt at length upon this point because so much has been written comparing the edge of a razor to that of a saw, that some persons actually advise us to give the edges of our knives and razors a saw-like character, under the impression that by so doing they will work better. This is a fallacy; the best work will be obtained from the smoothest and most perfect edge, provided the latter is thin enough.
If the material of which our cutting instruments are made would allow it, that is to say if it was perfectly hard, perfectly rigid and incapable of being crushed or torn apart, the proper shape of a cutting instrument would be a wedge of the most acute form possible. But since the steel which we use is limited in its hardness and its power of resisting crushing and bending influences, the blades of our knives must have a certain strength or thickness, depending upon the character of the material to be cut. For very hard substances the knife must be stout and the edge ground to a comparatively obtuse angle; for moderately soft substances, such as wood, the angle may be more acute, while for very soft tissues the thinnest blade and the sharpest edge are most suitable.
