The solution here referred to, and which is known as Kleinenberg’s solution, is made in the following way:

(1.) Make a saturated solution of crystallized calcium chloride in 70 per cent. alcohol, and add alum to saturation. (2.) Make also a saturated solution of alum in 70 per cent. alcohol. Add (1) to (2) in the proportion of 1:8. To the mixture add a few drops of a saturated solution of hæmatoxylin in absolute alcohol.

This solution may be used in very many cases for staining sections, in place of the ordinary watery solution of logwood alum. It may, if required, be diluted with the mixture of 1 and 2. The stained sections are placed at once in strong spirit.


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