The Crayfish: An Introduction to the Study of Zoology.

by Thomas Henry Huxley

If a thin vertical slice of the soft cuticle is gently {193} pulled with needles in the direction of its depth, it stretches to eight or ten times its previous diameter, the clear intervals between the dark bands becoming proportionally enlarged, especially in the middle of the slice, while the dark bands themselves become apparently thinner, and more sharply defined. The dark bands may then be readily drawn to a distance of as much as 1‐300th of an inch from one another; but if the slice is stretched further, it splits along, or close to, one of the dark lines. The whole of the cuticular layer is stained by such colouring matters as hæmatoxylin; and, as the dark bands become more deeply coloured than the intermediate transparent substance, the transverse stratification is made very manifest by this treatment.

Examined with a high magnifying power, the transparent substance is seen to be traversed by close-set, faint, vertical lines, while the dark bands are shown to be produced by the cut edges of delicate laminæ, having a finely striated appearance, as if they were composed of delicate parallel wavy fibrillæ.

In the calcified parts of the exoskeleton a thin, tough, wrinkled epiostracum (fig. [56], B, a), and, subjacent to this, a number of alternately lighter and darker strata are similarly discernible: though all but the innermost laminæ are hardened by a deposit of calcareous salts, which are generally evenly diffused, but sometimes take the shape of rounded masses with irregular contours.

Immediately beneath the epiostracum, there is a zone {194} which may occupy a sixth or a seventh of the thickness of the whole, which is more transparent than the rest, and often presents hardly any trace of horizontal or vertical striation. When it appears laminated, the strata are very thin. This zone may be distinguished as the ectostracum (b), from the endostracum (c), which makes up the rest of the exoskeleton. In the outer part of the endostracum, the strata are distinct, and may be as much as 1‐500th of an inch thick, but in the inner part they become very thin, and the lines which separate them may be not more than 1‐8000th of an inch apart. Fine, parallel, close-set, vertical striæ (e) traverse all the strata of the endostracum, and may usually be traced through the ectostracum, though they are always faint, and sometimes hardly discernible, in this region. When a high magnifying power is employed, it is seen that these striæ, which are about 1‐7000th of an inch apart, are not straight, but that they present regular short undulations, the alternate convexities and concavities of which correspond with the light and the dark bands respectively.

If the hard exoskeleton has been allowed to become partially or wholly dry before the section is made, the latter will look white by reflected and black by transmitted light, in consequence of the places of the striæ being taken by threads of air of such extreme tenuity, that they may measure not more than 1‐30,000th of an inch in diameter. It is to be concluded, therefore, that {195} the striæ are the optical indications of parallel undulating canals which traverse the successive strata of the cuticle, and are ordinarily occupied by a fluid. When this dries up, the surrounding air enters, and more or less completely fills the tubes. And that this is really the case may be proved by making very thin sections parallel with the face of the exoskeleton, for these exhibit innumerable minute perforations, set at regular distances from one another, which correspond with the intervals between the striæ in the vertical section; and sometimes the contours of the areæ which separate the apertures are so well defined as to suggest a pavement of minute angular blocks, the corners of which do not quite meet.

When a portion of the hard exoskeleton is decalcified, a chitinous substance remains, which presents the same structure as that just described, except that the epiostracum is more distinct; while the ectostracum appears made up of very thin laminæ, and the tubes are represented by delicate striæ, which appear coarser in the region of the dark zones. As in the naturally soft parts of the exoskeleton, the decalcified cuticle may be split into flakes, and the pores are then seen to be disposed in distinct areæ circumscribed by clear polygonal borders. These perforated areæ appear to correspond with individual cells of the ectoderm, and the canals thus answer to the so-called “pore-canals,” which are common in cuticular structures and in the walls of many cells which bound free surfaces. {196}

The whole exoskeleton of the crayfish is, in fact, produced by the cells which underlie it, either by the exudation of a chitinous substance, which subsequently hardens, from them; or, as is more probable, by the chemical metamorphosis of a superficial zone of the bodies of the cells into chitin. However this may be, the cuticular products of adjacent cells at first form a simple, continuous, thin pellicle. A continuation of the process by which it was originated increases the thickness of the cuticle; but the material thus added to the inner surface of the latter is not always of the same nature, but is alternately denser and softer. The denser material gives rise to the tough laminæ, the softer to the intermediate transparent substance. But the quantity of the latter is at first very small, whence the more external laminæ are in close apposition. Subsequently the quantity of the intermediate substance increases, and gives rise to the thick stratification of the middle region, while it remains insignificant in the inner region of the exoskeleton.

The cuticular structures of the crayfish differ from the nails, hairs, hoofs, and similar hard parts of the higher animals, insomuch as the latter consist of aggregations of cells, the bodies of which have been metamorphosed into horny matter. The cuticle, with all its dependencies, on the contrary, though no less dependent on cells for its existence, is a derivative product, the formation of which does not involve the complete {197} metamorphosis and consequent destruction of the cells to which it owes its origin.

The calcareous salts by which the calcified exoskeleton is hardened can only be supplied by the infiltration of a fluid in which they are dissolved from the blood; while the distinctive structural characters of the epiostracum, the ectostracum, and the endostracum, are the results of a process of metamorphosis which goes on pari passu with this infiltration. To what extent this metamorphosis is a properly vital process; and to what extent it is explicable by the ordinary physical and chemical properties of the animal membrane on the one hand, and the mineral salts on the other, is a curious, and at present, unsolved problem.

The outer surface of the cuticle is rarely smooth. Generally it is more or less obviously ridged or tuberculated; and, in addition, presents coarser or finer hair-like processes which exhibit every gradation from a fine microscopic down to stout spines. As these processes, though so similar to hairs in general appearance, are essentially different from the structures known as hairs in the higher animals, it is better to speak of them as setæ.