In order to obtain data to balance viability effects, the following experiment was made:

Vermilion (not-bar) fused males were bred to (red) bar (not-fused) females. The daughters and sons were bar. The daughters were back-crossed, singly, to vermilion fused males and gave the results shown in table 35. Each female was also transferred to a second culture bottle, so that for each female there are two broods given consecutively (82, 82′, etc.) in table 35.

The results given by the two broods of the same female are similar. The values are very near to those given in the last experiment, and confirm the conclusions there drawn. The combined data give the results shown in table 36.

Table 35.—P1 bar ♀ ♀ × vermilion fused ♂ ♂. B. C. F1 bar ♀ × vermilion fused ♂ ♂.

Reference.

Total. Cross-over values.
Ver-
milion
fused.		 Bar. Ver-
milion
bar.		 Fused. Ver-
milion.		 Bar
fused.		 Ver-
milion
bar
fused.		 Wild-
type.		 Ver-
milion
bar.		 Bar
fused.		 Ver-
milion
fused.
82 165 165 63 57 8 7 1 .. 466 263 29
82′ 104 87 26 24 .. 4 .. .. 245 202 22
83 128 164 51 39 6 4 .. .. 392 233 26
83′ 100 94 28 30 4 4 .. .. 260 223 25
89 85 105 23 24 5 2 .. .. 244 193 22
89′ 78 91 21 27 1 2 .. 1 221 222 23
90 86 85 30 28 5 .. .. .. 234 252 27
90′ 33 38 22 14 4 1 .. 1 113 335 36
91 125 107 41 31 1 1 .. .. 306 241 24
91′ 91 95 31 25 5 1 .. 2 250 233 25
92 109 136 41 24 4 2 .. .. 316 212 23
92′ 100 105 29 29 .. 1 .. 1 265 221 22
93 75 67 19 20 .. 1 .. .. 182 211 22
93′ 68 94 31 17 1 1 .. .. 212 231 24
94 84 96 31 35 8 1 .. .. 255 264 29
94′ 61 73 20 22 5 4 .. .. 185 235 28
95 84 102 27 26 3 3 .. .. 245 222 24
96 144 148 43 34 1 2 .. 1 373 211 21
97 81 96 25 20 5 3 .. .. 230 204 23
98 107 112 39 33 1 2 .. .. 294 251 26
Firsts 1,273 1,383 433 371 47 28 1 1 3,537 232 25
Seconds 635 677 208 188 20 18 .. 5 1,751 233 25
Total.1,908 2,060 641 559 67 46 1 6 5,288 23 2.3 25

Table 36.—Linkage of vermilion, bar, and fused with balanced viability.

Total.
5,621 1,756 175 15 7,567
Percentage 74.3 23.19 2.31 0.2

Some additional data bearing on the linkage of vermilion and fused were obtained. Males of (red) fused stock were bred to vermilion (not-fused) females, and gave wild-type females and vermilion males, which inbred gave the results shown in table 37.

The percentage of cross-overs between vermilion and fused is here 27, which is in agreement with the 26 per cent of the preceding experiment.