entirely absent. This result is due to a lethal factor, lethal 5, which appeared in this culture for the first time.

The males of the two cultures 149 and 151 give the order of gens as facet, vermilion, sable; that is, facet lies to the left of vermilion and toward yellow. The cross-over values are: facet vermilion 40; vermilion sable 12; facet sable 42. Since yellow and vermilion usually give but 34 per cent of crossing-over, this large value of 40 for facet vermilion shows that facet must lie very near to yellow.

Table 59.—P1 facet ♂ × vermilion sable ♀ ♀. B.C. F1 wild-type ♀ × vermilion sable ♂ ♂.

Reference. F2 females. F2 males.
Non-cross-overs. Cross-overs.

Ver-
milion
sable.
Wild-
type.
Ver-
milion.
Sable. Facet. Ver-
milion
sable.
Facet
ver-
milion
sable.
Wild-
type.
Facet
sable.
Ver-
milion.
Facet
ver-
milion.
Sable.
149 16 29 3 3 17 10 8 12 2 .. 2 1
150 13 17 2 2 .. 10 9 .. 1 .. .. ..
151 37 63 7 2 38 23 12 26 2 8 4 1
Total. 66 109 12 8 55 43 29 38 5 8 6 2

LINKAGE OF EOSIN, FACET, AND VERMILION.

In order to obtain more accurate information on the location of facet, a facet male was mated to an eosin vermilion female. The F1 females were mated singly to wild males and they gave the results shown in table 60. The F2 females were not counted, since they do not furnish any information. The evidence of table 60 places facet at 1.1 units to the right of eosin, or at 2.2.

Table 60.—P1 eosin vermilion ♀ × facet ♂. F1 wild-type ♀ × wild ♂.

Reference.

Total. Cross-over values.
Eosin
ver-
milion.
Facet. Eosin
facet.
Ver-
milion.
Eosin. Facet
ver-
milion.
Eosin
facet
ver-
milion.
Wild-
type.
Eosin
facet.
Facet
ver-
milion.
Eosin
ver-
milion.
512 43 43 .. 1 13 16 .. .. 116 .... .... ....
513 28 35 .. 2 19 5 .. .. 89 .... .... ....
514 18 31 1 .. 17 11 .. .. 78 .... .... ....
515 18 60 .. .. 20 15 .. .. 113 .... .... ....
516 10 31 .. .. 7 12 .. .. 60 .... .... ....
517 24 34 .. .. 10 12 .. .. 80 .... .... ....
518 44 38 1 1 23 22 .. 1 130 .... .... ....
Total. 185 272 2 4 109 93 .. 1 666 1.05 30.5 31.3

LETHAL SC.