When the insects are to be killed on the spot, we employ another and a very simple plan.

We take one of the old-fashioned wooden lucifer-match boxes, bore a hole in the lid, and push through the hole a swan-quill or the barrel of one of the swan-quill steel pens. A glass tube is still better, but is too fragile. Beeswax is tightly worked into the junction of the tube with the wood, so as to make it as nearly air-tight as possible. A cork stopper is then cut to fit the tube. The accompanying [illustration] will show the box completed. When this is finished, we take the smallest-sized pill-box, bore a number of holes in it with a red-hot needle, place a little piece of solid ammonia within it, and inclose it in the lucifer-box. Its effects are almost instantaneous; for scarcely has the insect touched the bottom of the box before it is helpless, and in a very few moments it is quite dead, so powerful is ammonia towards insects. The reader will of course understand that the pill-boxes must never have been used for pills, and that the match-box must be carefully cleaned before employing it in the microscopic service. Moreover, any boxes that have been used for lepidopterous insects become useless, inasmuch as the scales always fall from the wings, and cling to the sides of the box, so as to mix with succeeding objects, and very much puzzle the observer.

Aquatic and marine objects require bottles, and, as a general rule, these bottles ought always to have wide mouths. Indeed, if there be no shoulder at all, their purpose will be better served, as a small object is very apt to be caught under the shoulder, and to give much trouble before it can be removed without injury. Wide and short test-tubes answer admirably for collecting; and it will always be advisable to have a few small test-tubes ready fitted with corks, for the purpose of isolating those specimens which might receive or cause injury by being mixed with others.

To remove minute objects from one vessel into another is a very easy process. Take a glass tube, mark off a portion about eight inches in length, cut a little notch with a file, and bend it smartly, when it will break neatly across, without leaving points or having the regularity of its ends injured by gaps. Turn each end round and round in the flame of the spirit-lamp, and you have an ordinary “pipette.” The object of placing the ends of the tube in the flame is to render the edges quite smooth and rounded.

Now mark off the same length of tube, and place the marked portion in the flame, taking care to warm it well first, lest the sudden heat should crack the glass. Keep it continually turning between the fingers, and when it is quite soft, and of a fine red heat, draw the hands smartly apart, and you will produce a couple of tubes tapering to very fine points. Break off the tapering portions at any convenient point, round the edges as before, and you will then have pipettes suitable for small objects. As there are many specimens, especially the smaller animalculæ, which have a habit of retiring into the remotest corner, it is necessary to bend another pipette, so as to follow them. For our own part, we prefer the pipette to be bent nearly to a right angle.

The mode of using these simple instruments is as follows:—Place the forefinger or thumb firmly on the large end, and push the point under water. When the opening is close to the sought-for object, lift the finger suddenly, and admit the air into the tube. The water will immediately rush in at the lower end, and if the orifice has been properly directed, will carry the object into the tube. The finger is again applied to the mouth of the tube, and the object can be then carried off.

As with the pocket-lens almost every object is to be viewed by means of direct light, the young observer will find himself much aided by a suitable background. Any small object, such as a minute insect, a seed, or a hair, becomes very indistinct if held up against the light, or even when viewed against a broken background of trees, houses, or herbage. The simplest plan of securing a proper background is to take a disc of ivory, bone, or even of white cardboard, and to blacken one side of it. The black paint which is used for this purpose must be without gloss, and have what is called a “dead” surface. Ink answers very well for the purpose, and so does ivory-black; but Indian ink is too glossy to be serviceable.

To procure specimens from the water is a matter of some difficulty if managed badly, but easy enough when the collector knows his business. It is of course needful to attach the collecting vessel to the end of a rod, and to plunge it into the spots which look most favourable. Now even so simple a matter as this requires some little care, if the young microscopist really wishes to obtain the best specimens. A common walking-stick will answer most purposes; but the most efficient rod for the purpose is one of the common walking-stick fishing-rods without the top joint, as it can be carried without attracting attention, and can be lengthened at will by adding the different joints.