These experiments to test the effect of changing colors are also of interest in that they show in a remarkable way the influence of the direction of turning. The animal after succeeding in getting around the first part of the labyrinth failed entirely to escape at the exit. Here it should have turned to the left, instead of the right as it was accustomed to, but it persisted in turning to the right. Fig. 3 represents approximately the path taken in the first trial; it shows the way in which the animal persisted in trying to get out on the right. From this it is clear that both vision and the complex sensations of turning are important.
Fig. 3. Labyrinth with Conditions the Reverse of the Usual. (Compare with Fig. 2.) The colors as well as the partitions have been shifted. The path is, approximately, that taken by No. 2 in the first trial after the reversal of conditions.
The latter part of Table IV. presents further evidence in favor of vision. For these tests the colors alone were reversed. Previous to the change the animal had been making no mistakes whatever, thereafter there were four mistakes at the entrance and none at the exit. Later, another experiment under the same conditions was made with the same animal, No. 2, with still more pronounced results. In this case the animal went to the white, that is, in this instance, into the blind alley, and failed to get out; several times it jumped over to the left side (the open-passage side) of the box but each time it seemed to be attracted back to the white or repelled by the red, more probably the latter, as the animal had been trained for weeks to avoid the red. Concerning the delicacy of visual discrimination I hope to have something to present in a later paper.
The tactual stimuli given by contact with the series of wires used for the electrical stimulus also served to guide the frogs. They were accustomed to receive an electrical shock whenever they touched the wires on the blocked side of the entrance, hence on this side the tactual stimulus was the signal for a painful electrical stimulus. When the animal chose the open passage it received the tactual stimulus just the same, but no shock followed. After a few days' experimentation it was noted that No. 2 frequently stopped as soon as it touched the wires, whether on the open or the closed side. If on the closed side, it would usually turn almost immediately and by retracing its path escape by the open passage; if on the open side, it would sometimes turn about, but instead of going back over the course it had just taken, as on the other side, it would sit still for a few seconds, as if taking in the surroundings, then turn again and go on its way to the exit. This whole reaction pointed to the formation of an association between the peculiar tactual sensation and the painful shock which frequently followed it. Whenever the tactual stimulus came it was sufficient to check the animal in its course until other sensory data determined the next move. When the wrong passage had been chosen the visual data gotten from the appearance of the partition which blocked the path and other characteristics of this side of the labyrinth determined that the organism should respond by turning back. When, on the other hand, the open passage had been selected, a moment's halt sufficed to give sensory data which determined the continuation of the forward movement. Although this reaction did not occur in more than one tenth of the trials, it was so definite in its phases as to warrant the statements here made. Fig. 4 gives the path taken by No. 2 in its 123d trial. In this experiment both choices were correctly made, but when the frog touched the wires on the open side it stopped short and wheeled around; after a moment it turned toward the exit again, but only to reverse its position a second time. Soon it turned to the exit again, and this time started forward, taking a direct course to the tank. The usual course for animals which had thoroughly learned the way to the tank is that chosen in Fig. 5.
Fig. 4. Path of No. 2 for 123d Trial. Showing the response to the tactual stimulus from wires.
An interesting instance of the repetition of a reaction occurred in these experiments. A frog would sometimes, when it was first placed in the box, by a strong jump get up to the edge; it seldom jumped over, but instead caught hold of the edge and balanced itself there until exhaustion caused it to fall or until it was taken away. Why an animal should repeat an action of the nature of this is not clear, but almost invariably the second trial resulted in the same kind of reaction. The animal would stop at the same point in the box at which it had previously jumped, and if it did not jump, it would look up as if preparing to do so. Even after a frog had learned the way to the tank such an action as this would now and then occur, and almost always there would follow repetition in the manner described.
Fig. 5. Path Usually Taken by Animal Having Perfectly-formed Habit.