The auditory stimulus used was either the sound of a quick hammer blow (momentary stimulus of Series I), or the ringing of an electric bell for a certain length of time (prolonged stimulus of Series II). In Fig. 1 the bell is shown. It was placed eighty cm. from the frog, and in order that the influence of vibration of the experiment table might be avoided it was suspended from the pendulum frame. When the hammer was used it was placed sixty cm. from the frog, on the pendulum table. The holder for the frog and the tactual apparatus occupied a separate table which was not disturbed by the jars of the pendulum table.
Figure 1. Auditory-tactual Reënforcement-Inhibition apparatus. P, pendulum; p, contact point of P; b, attachment for electro-magnet, a; m, key for circuit of electric bell, B; n, key for magnet circuit of tactual apparatus; K, hand-key for release of pendulum and temporary closing of electric bell circuit; k1, k2, k3, keys in circuits; e, f, g, magnetic release for tactual apparatus; l, pivoted lever, bearing rubber cone, T, and weights, w. (Drawn by Dr. Wm. E. Hocking.)
The tactual stimulus was given by a rubber cone, T, two mm. in diameter at its apex. This rubber point, after the electric release of the lever to which it was attached, struck the frog at the middle point of a line drawn between the posterior margins of the tympana. The intensity of the stimulus could be varied by weighting the lever, l, at w.
All experiments were made with the green frog, Rana clamata Daudin. The reactions were taken regularly at half-minute intervals in pairs: first, a tactual stimulus reaction, then an auditory-tactual reaction. Ten, fifty, or one hundred pairs constituted a series. So far as the condition of the frog is concerned there seems to be nothing undesirable in long series, for fatigue does not appear, and so long as the animal is kept moist and in an unconstrained position, it continues to react normally, and without frequent struggles to escape. The advantage for the purposes of this investigation of taking the reactions in pairs, rather than taking separate series of reactions for each stimulus or combination of stimuli, is obvious. It enables us to compare directly the reactions of each pair, in other words those reactions which took place under most nearly identical conditions, and to note at once whether the auditory stimulus reënforced or inhibited the tactual reaction.
During a series the intensity of the tactual stimulus was changed as conditions demanded, but for any one pair of reactions it was always the same. It not infrequently happened that an intensity which at first caused merely a slight movement of the leg, later in the series uniformly brought about a maximal contraction, or the reverse might be true, and inasmuch as a maximal reaction to the tactual stimulus alone left no opportunity for judging of the influence of the auditory stimulus, when it was given in addition to the tactual, it was always necessary in such cases so to alter the intensity of the tactual stimulus that a medium reaction resulted.
The frogs, after being placed in the saddle-like holder and held firmly for a few seconds, seldom struggled very much, but if bound tightly they became irresponsive to the stimuli.[154] It was, therefore, necessary after they had quieted down to loosen the bands which held them in position. For the purpose of excluding the influence of visual stimuli a wire screen cap covered with black cloth was put over the head; this served to keep the animal in position as well as to exclude visual stimulation.
a. Momentary auditory stimulation. Four frogs were used for a study of the influence of the momentary sound produced by a hammer blow, and for each of these animals fifty pairs of reactions were recorded in series each day. The temporal relation of the stimuli was changed daily during a week of experimentation: the results therefore consist of fifty pairs of reactions with each frog for each of the following seven intervals: (1) Auditory and tactual stimuli simultaneous, (2) auditory .25˝ before tactual, (3) auditory .45˝ before, (4) auditory .15˝ before, (5) auditory .65˝ before, (6) auditory .35˝ before, (7) auditory .90˝ before. The intervals were used in the experiments in the above order to avoid the formation of the definite habits of reaction which regular increase in the interval would have favored.