It was soon found that a great deal depended on the method of experimentation. None of the experimenters mentioned above gives any detailed description of the manner in which the experiments were carried out. One is left uncertain whether the animals were placed on the periphery of the turn-table or over the centre, whether in the former case they were set with their heads toward the centre or away from it, or placed at right angles to a radius, or whether they were merely set down in any chance fashion and whirled about. The same indefiniteness exists in most of the accounts as to how fast they were turned, and whether the experiments were performed in the air or in the water. Finally it is not stated whether the rotation was always in the same direction, or whether its direction was alternated.

The turn-table used in the following experiments was one that had to be turned by hand, so that it was impossible to regulate the speed accurately. The crank, however, was not attached directly to the rotating board, but was connected with it by means of a gearing so that one turn of the crank produced about ten turns of the table. This gearing gave a steadying effect to the motion so that the speed could be kept tolerably constant. A circular pan, about 15 cm. in diameter at the bottom with the sides slightly sloping outward, was set so that its centre coincided with the axis of the rotating table. It was in this pan that all the experiments were tried. Through various preliminary experiments to determine the most favorable speed, it was found that a rotation rate of over one turn of the table per second produced such a strong centrifugal force that unless the animals were set exactly over the centre they were swept off against the side of the pan in such a manner that it was difficult to decide whether the rotation as such had any effect upon their movements. It was finally decided that the best results were obtained from a rate of approximately one rotation in two seconds.

It soon became evident that when the larger and more sluggish crayfish were merely dropped in the pan and rotated there was no particular reaction. This was true whether the animals rotated were in the air or in the water. The smaller and more active crayfish, however, showed a decided tendency to run either with or against the direction of the rotation, especially when the experiments were carried on in the water. In no case was there any tendency to go in the opposite direction when the rotation ceased, except in so far as the animals were carried along by the water. To get a quantitative expression for these tendencies a more delicate method of experimentation was resorted to. If there was a tendency on the part of the active animal to move either with or against the rotation, such a tendency might also be supposed to exist in the sluggish animal, only in the latter the inertia was sufficiently strong to prevent its appearance. If, however, the animals should be set radially to the periphery of the pan, the tendency to go with or against the rotation would be exhibited in the direction in which they turned out of the radial position. For it was found that no animal would remain in that position for any great length of time. Two groups of animals were used for these experiments, five animals in each group, and the first group was selected from the smallest and most active animals, the second from the largest and most sluggish. Each animal was set in two positions, position I, with the head toward the centre, position II, with the head away from the centre. Each animal was given ten trials in each position, and the number of times it turned in a direction with the rotation is set down in the + column, the number of times it turned against the rotation is indicated in the - column. In general from 5 to 15 turns were necessary for the orientation of the animal, though sometimes the number ran up to 30 or 40. Each trial was made in the opposite direction to the preceding one, in order to avoid the formation of any habit in turning. All these experiments were carried out in water, the depth of which in the pan was about 4 cm. In order that there should be no difference between the velocity of the water and that of the pan, the table was rotated a few times before the animal was put in. As a check a series of experiments of 5 in each position was performed in the air on the more active group.

The following table shows the results of these experiments in rotation:

TABLE VIII. REACTIONS TO ROTATION

In Water In Air
I II Sum I II Sum Sum Total
Group 1+– +– +– +– +– +– +–
4455 10 515 14 5 19 624
4928 46 614 5 14 19 723
5637 82 119 5 32 82 1911
6246 10 146 23 23 46 1812
6428 91 119 14 14 28 1317
Sum1634 3119 4753 916 718 1634 6387
Group 2
2155 37 812
2728 55 713
3673 82 155
3728 37 515
5455 64 119
Sum2129 2525 4654 4654
Sum Total3763 5644 93107 916 718 1634 109141

Examination of the table reveals great individual variation. Some animals, as nos. 44, 49, and 37, turn rather constantly against the direction of the rotation, while others, as nos. 56 and 36, are almost as constant in their movement with the rotation. On the whole we observe that for each group, and for Group 1 in both water and air, there is a slightly greater tendency to go against the rotation than with it. This tendency, strange to say, comes out much more clearly in the air than in the water. It is evident, however, from the variation exhibited that there is nothing very stereotyped or mechanical about the reaction. Mention should be made of the fact that usually (though not always) the animals not only oriented themselves with reference to the rotation, but moved forward in that direction as long as the rotation continued.

IV. GEOTAXIS, BAROTAXIS, AND TURNING