Passing on to the leaves, we may note the importance of obtaining in every case the root-leaves of each species. These are often very different in form from the stem-leaves, as in such species as the harebell (Campanula rotundifolia), Pimpinella saxifraga, the earth-nut (Bunium flexuosum), and many more; in some instances, as in the Jersey bugloss (Echium plantagineum), they at once characterize the species. Still more important are these lower leaves in the case of water-plants: in the arrowhead (Sagittaria), for example, they are narrow, and resemble those of the bur-reed (Sparganium); and in the water plantain (Alisma plantago), the submerged leaves are equally different from those which rise out of the water. This difference is still more noticeable in the case of the aquatic Ranunculi, where a knowledge of the submerged leaves is essential to the discrimination of the various forms or species.
Where practicable, the whole plant should be collected for the herbarium; but when, from its size, this cannot be accomplished, leaves from the root, the centre of the main stem, and the lateral branches, should be taken. As to the stem itself, that must be represented: in the Rubi, indeed, it is essential. "To judge properly of a bramble from a preserved specimen," says Professor Babington, "we require a piece of the middle of the stem with more than one leaf; the base and tip of the stem are also desirable, likewise a piece of the old stem with the flowering shoot attached to it; the panicle with flowers, and the fruit. We likewise want to know the direction of the stem throughout, of the leaflets, and of the calyx; also the shape of the petals and the colour of the styles: a note of these should be made when the specimen is gathered."
Passing on to the flowers, we shall find it necessary to represent them in almost every stage, from the bud to the perfecting of the fruit. It is of course in most cases possible to select an example in such a state as to show upon the same plant buds, flowers, and fruits; but where this is not the case, each of these particulars must be supplemented by additional specimens. The turn which botanical investigation has recently taken towards the study of the phenomena connected with fertilization has given the collector another subject to which his attention may be profitably directed. It has been observed that in some plants the stamens are developed before the pistils; in others, the pistils are matured before the stamens; while in yet a third set, stamens and pistils are simultaneously perfected. These three groups of plants are termed respectively protandrous, protogynous, and cynacmic, and a very little observation will show that examples of each are sufficiently common.
Then in diœcious and monœcious plants, both male and female flowers must be collected, and in some cases, as in the willows, four specimens are necessary to the complete presentment of the species, showing respectively the male and female catkins, the leaves, and the fruit. Some plants produce two distinct forms of blossom, as is noticeable in the violets and the woodsorrel, one being conspicuous and usually barren, the other insignificant and often apetalous, but producing perfect fruit. The pollen will afford occupation to the microscopist: the researches of Mr. Gulliver and Mr. Charles Bailey have demonstrated that important distinguishing characters are in some instances furnished by it. While on this point it may be suggested that it is convenient in many cases to collect several specimens of the flowers alone, which, when dried, should be placed in a small envelope or capsule, and attached to the sheet on which the plant is represented. In the event of any examination which may be requisite after the plant is dried, these detached blossoms will be found very useful, and will prevent the necessity of damaging the specimen. In the case of such plants as shed their corollas very readily, as the speedwells, it is as well to put them in press as soon as collected; and the colour of many may be retained by the same means.
The fruits and seeds of plants are too generally neglected by amateur collectors, but are essential to the completeness of a specimen. It may be found practically convenient to keep these in a separate place, and detached from the plant; and in many cases of dried fruits it is advisable to sort them into their places without previous pressing. By this means the modes of dehiscence will readily be seen: pulpy and succulent fruits should be preserved in spirit. In such plants as the species of sea sandwort (Lepigonum), and some Chenopodia, important specific characters are drawn from the seed; as they are from the pods of Melilotus and the fruits of Agrimonia. In collecting ferns, well-fruited fronds must be selected, as it is impossible to determine specimens without fructification. Grasses should be selected when in flower and fruit, but must not be allowed to attain too great an age before they are collected.
We have been speaking so far of the things to be collected, and space will not allow us to dilate at any length upon the apparatus necessary to that end. Nor indeed is this necessary; a good-sized vasculum, with one or two smaller boxes for the pocket, in which the more delicate plants may be preserved; a strong pocket-knife or small trowel, for procuring roots, and a hooked stick wherewith to fish out water-plants, or pull down branches, are the principal things required. To anyone residing for any length of time, or even only for a few days, in a district, a "London Catalogue" is an important acquisition, in which should be marked off all the species met with; by this means the flora of the neighbourhood is ascertained at a very slight expenditure of time and trouble. It is not advisable to collect too many plants at once, or to crowd the vasculum, unless under exceptional circumstances; nor should the desire to possess rare plants tend, as is too often the case, to the neglect and exclusion of commoner ones.
A careful and observant collector will frequently meet with forms which deviate more or less from the accepted type of a species. When these appear to offer any marked characters, they should be noted; and in all cases it is well to preserve any forms which, from external circumstances, have a different appearance from the normal state. The differences produced by soil and situation alone are very considerable; and though the essential characters are usually to be discerned, the interest and value of a herbarium are very much increased by a selection of examples showing the range of a species. Campanula glomerata offers a good example of this. In damp meadows it is from one to two feet high, with a large spreading terminal head of blossoms, while on chalk downs it does not attain more than as many inches, with only one or two flowers; in this state it was described by Withering as a gentian, under the name of Gentiana collina; and the same author gives as Campanula uniflora a one-flowered mountain state of the harebell (C. rotundifolia).
The collector will also do well to keep a look-out for deviations in structure, which are often of great interest. In short, nothing should be neglected which can tend to the perfect presentment of a species in the herbarium: its utility is commensurate with its completeness. The mere collector may be satisfied with scraps of a rare plant and the absence of commoner species; but the real worker will pride himself rather upon the possession of instructive examples, which may be of assistance to himself, as well as to all those who may consult them.