The resting neural membrane is thus polarized, with the inside approximately 60 millivolts negative with respect to the outside. Most of the Hodgkin-Huxley data is based on measurements of the transmembrane current in response to an imposed stepwise reduction (depolarization) of membrane potential. By varying the external ion concentrations, Hodgkin and Huxley were able to resolve the transmembrane current into two “active” components, the potassium ion current and the sodium ion current. They found that while the membrane permeabilities to chloride and most other inorganic ions were relatively constant, the permeabilities to both potassium and sodium were strongly dependent on membrane potential. In response to a suddenly applied (step) depolarization, the sodium permeability rises rapidly to a peak and then declines exponentially to a steady value. The potassium permeability, on the other hand, rises with considerable delay to a value which is maintained as long as the membrane remains depolarized. The magnitudes of both the potassium and the sodium permeabilities increase monotonically with increasing depolarization. A small imposed depolarization will result in an immediately increased sodium permeability. The resulting increased influx of sodium ions results in further depolarization; and the process becomes regenerative, producing the all-or-none action potential. At the peak of the action potential, the sodium conductance begins to decline, while the delayed potassium conductance is increasing. Recovery is brought about by an efflux of potassium ions, and both ionic permeabilities fall rapidly as the membrane is repolarized. The potassium permeability, however, falls less rapidly than that of sodium. This is basically the explanation of the all-or-none spike according to the Modern Ionic Hypothesis.
Figure 5—Hodgkin-Huxley representation of small area of axon membrane
Figure 6—Typical responses of sodium conductance and potassium conductance to imposed step depolarization
By defining the net driving force on any given ion species as the difference between the membrane potential and the equilibrium potential for that ion and describing permeability changes in terms of equivalent electrical conductance changes, Hodgkin and Huxley reduced the ionic model to the electrical equivalent in [Figure 5]. The important dynamic variables in this equivalent network are the sodium conductance (G{Na}) and the potassium conductance (G{K}). The change in the sodium conductance in response to a step depolarization is shown in [Figure 6B]. This change can be characterized by seven voltage dependent parameters:
1. Delay time—generally much less than 1 msec
2. Rise time—1 msec or less
3. Magnitude of peak conductance—increases monotonically with increasing depolarization
4. Inactivation time constant—decreases monotonically with increasing depolarization.