(3) Boiling in water for 1 hour. This temperature does not destroy the spores of Bacillus subtilis in the time mentioned, but is effective for the destruction of the spores of the anthrax bacillus, and of all known pathogenic organisms.
(4) Chloride of lime: a solution of 4 in 100 containing at least 25 per cent. of available chlorine.
(5) Mercuric chloride: a solution of 1 in 500 containing at least 3 per cent. of available chlorine.
For the destruction of infectious material which owes its infecting power to the presence of micro-organisms not containing spores:—
(1) Fire: Complete destruction by burning.
(2) Boiling water ½ an hour.
(3) Dry heat: 230°F. for 2 hours.
(4) Chloride of lime: 1 to 4 in 100 solution, containing at least 25 per cent. available chlorine.
(5) Solution of chlorinated soda: 5 to 20 in 100 solution, containing at least 3 per cent. available chlorine.
(6) Mercuric chloride: a solution of 1 in 1000 to 1 in 4000.