There was now no alternative but the sword, and both parties at once took action accordingly. In support of the French troops which were at her disposal, the Regent ordered levies from Clydesdale, Stirlingshire, and the Lothians to meet her at Stirling on May 24th. On their part the insurgents strengthened the defences of Perth—according to Buchanan, the only walled town in Scotland—and addressed themselves to their brethren in Ayrshire for instant succor. As they were now engaged in what might be construed as rebellion, they took steps to justify themselves in the eyes of the world. In three manifestoes, probably the work of Knox, they addressed respectively the Regent, D'Oysel, the French ambassador, and the whole Scottish nobility. In view of the past history of Scotland the insurgents could present a case which possessed sufficient plausibility. It had been the exception for the reign of a Scottish king to pass without some more or less serious revolt on the ground of his alleged misgovernment. Even during the reign with which we are dealing, there had been a fair precedent for the late proceedings of the Congregation. At the outset of the reign, the Earl of Arran was giving away the country to England and to heresy; Beaton and the French party had taken up arms against him, and undone all his actions to which they objected. But as Mary of Lorraine was now governing the country, the danger of a French conquest was much more serious than had been the danger of conquest by England. On the ground that the state was in peril, therefore, there was ample justification for the action of the Protestant leaders. With regard to religion, the good of the commonwealth might easily be urged as a plea for the most drastic dealing with the national Church. By the admission of its own officials the Church had become a scandal, alike from the character of the clergy and its general neglect of its duties as a spiritual body. For at least a century the scandal had been growing; and good citizens had been forced to the conclusion that their accredited spiritual guides were either unable or unwilling to set their house in order.

But the time demanded deeds more than words. With a force of about eight thousand French and Scots, D'Oysel, the Regent's chief adviser, advanced to Auchterarder, some twelve miles from Perth. With this formidable force behind her, the Regent naturally expected that her rebellious subjects would be disposed to abate their demands. To learn what terms they would now be willing to accept, she sent to Perth the lord James Stewart, Lord Sempill, and the Earl of Argyle. They were told that the town would be surrendered if assurance were given of freedom of worship and security to the worshippers. As a reply to these demands, the Regent despatched the lyon king-of-arms to make proclamation that all should "avoid the toune under pane of treasone." At this moment, however, the Earl of Glencairn, at the head of a body of two thousand five hundred Ayrshire Protestants, made his way to within six miles of Perth. Thus checkmated, the Regent was again driven to a compromise; and on the conditions that she should quarter no French troops in the town, and grant perfect freedom of worship, the gates were at length thrown open to her. Thus closed the first act of the drama of the Scottish Reformation.

This good understanding was of short duration. Again the action of the Regent gave rise to an accusation of broken pledges. She kept to the letter of the late compact, but she evaded its spirit. She did not quarter French troops in the town, but she occupied it with Scottish soldiers in French pay, and, in further disregard of her pledges, treated the Protestants with a harshness which gave rise to bitter complaint on the part of their leaders. Argyle and the lord James, the two most prominent of these leaders, had accompanied her into Perth (May 29th), but, indignant at these proceedings, they secretly quitted the town and at once took action to make good their protests. Summoning the Protestant gentlemen of Angus and the Mearns to meet them in St. Andrew's on June 3d, they proceeded to that town, as the best centre of action after Perth. In St. Andrew's as in Perth it is John Knox who is again the outstanding figure. Here his preaching was attended by the same notable results. The monasteries of the Dominicans and the Franciscans were practically demolished by the mob, and with the approval of the magistrates every church in the town was stripped of its ornaments. Meanwhile the Regent had not been idle, and was now at Falkland with a force led by D'Oysel and Châtelherault. Confident in their strength, those two leaders marched toward Cupar, with the intention of dealing with St. Andrew's. But again they discovered that they had miscalculated the resources of the insurgents. Issuing from St. Andrew's, with little over a hundred horse, Argyle and the lord James were speedily reënforced by contingents from Lothian and Fife, which raised their numbers to above three thousand men. Thus strengthened, they took up their position on Cupar Muir, and awaited the approach of the Regent's forces. But in number these forces were now inferior to those of the enemy; and, as many of the French soldiers were Huguenots and secretly sympathized with their fellow-believers, the issue of the battle could not but be doubtful. Again, therefore, there was no alternative for the Regent but to temporize. It was agreed that there should be a truce of eight days, that the Regent's forces now in Fife should be removed from that county, and that, during the armistice, an attempt should be made to effect some permanent understanding.

The new arrangement proved as hollow as the first. In point of fact, it was borne in on both parties that the struggle had but begun, and that the sword only could end it. Already, therefore, both were looking for external support wherewith to crush their opponents. The very day after the compact at Cupar, D'Oysel wrote to the French ambassador in London that only a body of French troops could maintain the Regent's authority. On their part the Protestant leaders now entered on those negotiations with England which eventually led to results that gave Scotland definitely to Protestantism and united the destinies of the two nations. Meanwhile, however, the Regent and her revolted subjects had to fight their own battles. The truce effected nothing, and it had no sooner expired than hostilities recommenced. The first object of the leaders of the Congregation was to relieve their brethren in Perth, and on June 24th they sat down before that place in such numbers that it immediately and unconditionally surrendered. Perth, Dundee, and St. Andrew's were now in their hands; but, having gone thus far, their only hope lay in giving still further proof of the strength of their cause. It was reported that the Regent meant to stop their progress southward of Stirling bridge; but, before she could effect her object, they entered that town with the consent of the majority of the citizens. By June 29th they were in possession of the capital, whence Mary of Lorraine had fled to the castle of Dunbar.

The cause of the Congregation now appeared to be triumphant, but it contained elements of weakness of which everyone was aware and which speedily became manifest. The acts of violence which had attended the revolt were filling the law-abiding citizens with dismay. The destruction of church property in Perth and St. Andrew's had been followed by similar excesses elsewhere. Especially disquieting had been what had occurred at Scone immediately after the surrender of Perth. In defiance of the protests of Knox, the lord James, and Argyle, the reformers of Dundee had sacked and burned to the ground the abbey and palace of that village—an outrage which Knox himself regretted in the interest of his own cause. It was a further source of weakness to the Congregation that their actions easily lent themselves to misconstruction and misrepresentation. The Regent industriously spread the plausible report both at home and abroad that their religious professions were a mere pretext, and that their real object was to overthrow herself and to make the lord James their king. But, above all, the nature of the host that supported them was such that it invariably failed them when their need was the greatest. The men who composed it had to leave their daily business in town and country; and, as they received no pay and their own affairs demanded their attention, their military service did not extend beyond a few weeks. The Protestant leaders had no sooner taken possession of Edinburgh than their following began to dwindle. During the first week their numbers amounted to over seven thousand men; by the third week they had diminished to one thousand five hundred. In these circumstances the Regent had only to bide her time, and her opportunity must come. On July 23d her troops, led by D'Oysel and Châtelherault, marched on Leith, which they reached on the morning of the 24th. As had been anticipated, neither that town nor the capital itself was in a position to offer any effectual resistance; and the leaders of the Congregation at once proposed a conference for the discussion of terms. Accordingly, the Duke and the Earl of Huntly on the one side, and Argyle, the lord James, and Glencairn on the other, met on the east slope of the Calton hill and agreed to the following adjustment: The Congregation were to give up the coining-irons, of which they had taken possession, and they were to evacuate Edinburgh within twenty-four hours. The town was to be left free to choose its own religion; no French troops were to be introduced. The Protestants were to be allowed complete liberty of worship, but were to abstain from violence against the old religion, and these arrangements were to hold till the 10th of the following January. By this concession of liberty to worship according to their own consciences the Protestants had apparently attained the main object for which they had risen, but they well knew that they would enjoy this liberty only so long as they were strong enough to enforce it. On leaving Edinburgh, therefore, they proceeded to Stirling, where they came to an agreement as to their future plan of action. As a necessary precaution for their immediate security, they entered into a bond of mutual defence and concerted counsels. Above all, they determined to spare no pains to win support from England, which, as itself now a Protestant country, could not look on with indifference while they were engaged in a life-and-death struggle with France and Rome.

An event that had lately happened gave a new impulse to French action in Scotland. On July 10th Henry II had been accidentally killed in a tournament; and Mary Stuart, the niece of the Guises, was now Queen of France. It was with greater zeal than ever, therefore, that the Guises sought to direct Scottish affairs according to their own interests. In the beginning of August the Protestant lords took a decided step: they sent John Knox to England with instructions that might serve as a basis of a treaty between England and the Congregation. The instructions were that if England would assist them against France, the Congregation would agree to a common league against that country. Knox only went as far as Berwick; but he brought home a letter containing a reply to the Protestant overtures from Elizabeth's secretary, Sir William Cecil. The reply was discouraging; but it contained a practical suggestion, by which, however, the Protestant leaders were either unwilling or unable to profit. If it was money they were in need of, Cecil told them, that need present no difficulty; if they would but do as Henry VIII did with the monasteries, they would have enough money and to spare. The English Queen was, in truth, in a position that demanded the wariest going. Two-thirds of her own subjects were Catholics, and it would be an evil example to set them if she were to assist rebels in another country. Moreover, the treaty of Cateau-Cambrésis, concluded in the previous April, debarred her from hostile demonstration against France. But the peril from French ascendency in Scotland could not be ignored, and by the gradual pressure of events Elizabeth was driven to support a course which in her heart she abhorred. Shortly after Cecil's communication, the veteran diplomatist, Sir Ralph Sadler, came down to Scotland with a commission to effect a secret arrangement with the Protestant leaders, and brought with him three thousand pounds to distribute to the best of his wisdom.

What the Guises meant speedily became apparent. About the middle of August a thousand French soldiers landed at Leith; and, as they were accompanied by their wives and children, the object of their coming could not be misunderstood. If the leaders of the Congregation, therefore, were not to lose all the ground they had lately gained, a time for vigorous action had again come. As had been previously concerted, they met at Stirling on September 10th and took counsel as to their further action. Here they were joined by an ally who, by his rank and his claims, was of the first importance to their cause. This was the Earl of Arran, the eldest son of the Duke of Châtelherault, who, a few months previously, had been forced to flee from France by reason of his Protestant sympathies. The value of the new confederate was soon realized. Passing to Hamilton palace, the insurgent leaders there met the Duke himself, to whom they held out such alluring prospects that he openly identified himself with their cause. During these transactions at Hamilton, alarming news came of the doings of the Regent. It was reported that she was busily engaged in fortifying Leith—a proceeding, the Congregation maintained, in direct violation of the late treaty. Disregarding their protest, she steadily proceeded with the work; and, as she was strengthened by a new contingent of eight hundred French men-at-arms, her position by the middle of autumn was such as to excite alarm alike in Scotland and England. Again there was no arbitrament but by the sword.

On October 16th the insurgent leaders entered Edinburgh with the intention of laying siege to Leith, where the Regent had taken refuge as the safest place in the kingdom. One of their earliest steps was the most audacious they had yet taken. They formally deposed Mary of Lorraine from the regency, on the ground that she had ruled as a tyrant and was betraying the country to a foreign enemy. But they soon found that they had taken a task beyond their strength. Their force amounted to but eight thousand men, most of whom were "cuntrie fellows" with no experience in war, and whose service could not extend beyond a few weeks. To this undisciplined host was opposed a garrison of three thousand trained soldiers, with the command of the sea and intrenched in a town fortified after the best military art of the time. Fortune, moreover, was against the Congregation from the first. A new instalment of one thousand pounds, secretly sent by Elizabeth, was cleverly seized by James, Earl of Bothwell, afterward the notorious helpmate of Mary Stuart. Their arms, also, met with no success. While a detachment of their troops was in pursuit of Bothwell, the enemy found their opportunity and made their way even into the streets of Edinburgh; and on November 25th the reformers sustained so severe a reverse that the capital was no longer a safe place for them. They had no money to pay the few mercenaries whom they had hired; the town was tired of them; and the earl Marischal, who had charge of the castle, held resolutely aloof.

As at the close of their previous rising, the leaders held a council at Stirling to determine their future policy; before they entered on their deliberations, Knox was called upon to preach a sermon—Knox, of whom it was said that he "put more life" into those who heard him "than five hundred trumpets continually blustering" in their ears. The deliberations that succeeded took a sufficiently practical shape. Young Maitland of Lethington, who had lately deserted the Regent for the Congregation, was despatched to England with offers that might induce Elizabeth to give direct support to the cause of Protestantism in Scotland. As to their own future action, the lords made the following arrangement: Châtelherault, Argyle, Glencairn, and the lords Boyd and Ochiltry were to make their head-quarters in Glasgow; while Arran, the lord James, the lords Rothes and Ruthven, and John Knox were to act from St. Andrew's as their centre. Their counsels at an end, they separated with the intention of reassembling at Stirling on December 16th. They had thus tried two falls with the Regent, and in both they had been worsted: the third trial of strength was to have a different ending.

The Regent was not slow to follow up her advantage. She took possession of the capital two days after the Congregation had quitted it, and she tried hard, but in vain, to persuade the earl Marischal to surrender the castle. The arrival of fresh reënforcements from France at the beginning of December enabled her to abandon her defensive policy and to take decisive measures for the suppression of revolt. On Christmas Day, while the Protestant lords were in council at Stirling, two detachments of her troops, commanded by D'Oysel, drove them precipitately from the town. Pursuing his advantage, D'Oysel despatched his troops across Stirling bridge into Fife, and he himself with another detachment crossed from Leith, apparently with the object of gaining possession of St. Andrew's. The task proved a hard one. At every step he was beset by the Scots under Argyle and the lord James. "The said Earl and Lord James," says Knox, "for twenty-one days they lay in their clothes; their boots never came off; they had skirmishing almost every day; yea, some days, from morn to even." Yet, in the teeth of all obstacles, D'Oysel steadily forced his way to within six miles of St. Andrew's, where Knox and his friends had all but abandoned hope. But unexpected deliverance was at hand. On January 23, 1560, a fleet of strange vessels appeared at the mouth of the Frith of Forth. As a French fleet had been expected for some weeks, D'Oysel concluded that his armament had come at last. He was soon undeceived. Under his eyes the strangers seized two ships bearing provisions from Leith to his own camp. The strange vessels were an advanced squadron of a fleet sent by Elizabeth to block the Frith of Forth against further succors from France. It was now D'Oysel who was in extremities; and before he found himself safe in Linlithgow he had vivid experience at once of the rigors of a Scotch winter and of the savage hate which his countrymen had come to inspire in the nation which for three centuries had called them friends and allies.