These personal merits and this political necessity were the only pleas advanced in a letter to her ambassador in England. But that neither plea would avail her for a moment in Scotland she had ominous evidence on the thirteenth day after her marriage, when no response was made to the usual form of proclamation for a raid or levy of forces under pretext of a campaign against the rievers of the border. On June 6th Mary and Bothwell took refuge in Borthwick castle, twelve miles from the capital, where the fortress was in the keeping of an adherent whom the diplomacy of Sir James Melville had succeeded in detaching from his allegiance to Bothwell. The fugitives were pursued and beleaguered by the Earl of Morton and Lord Hume, who declared their purpose to rescue the Queen from the thraldom of her husband. He escaped, leaving her free to follow him or to join the party of her professed deliverers.

But whatever cause she might have since marriage to complain of his rigorous custody and domineering brutality was insufficient to break the ties by which he held her. Alone, in the disguise of a page, she slipped out of the castle at midnight, and rode off to meet him at a tower two miles distant, whence they fled together to Dunbar. The confederate lords on entering Edinburgh were welcomed by the citizens, and after three hours' persuasion Lethington, who had now joined them, prevailed on the captain of the castle to deliver it also into their hands. Proclamations were issued in which the crime of Bothwell was denounced, and the disgrace of the country, the thraldom of the Queen, and the mortal peril of her infant son were set forth as reasons for summoning all the lieges of the chief cities of Scotland to rise in arms on three hours' notice and join the forces assembled against the one common enemy. News of his approach reached them on the night of June 14th, and they marched before dawn with twenty-two hundred men to meet him near Musselburgh. Mary meanwhile had passed from Dunbar to Haddington, and thence to Seton, where sixteen hundred men rallied to her side. On June 15th, one month from their marriage day, the Queen and Bothwell, at the head of a force of fairly equal numbers but visibly inferior discipline, met the army of the confederates at Carberry hill, some six miles from Edinburgh.

It was agreed that the Queen should yield herself prisoner, and Bothwell be allowed to retire in safety to Dunbar with the few followers who remained to him. Mary took leave of her first and last master with passionate anguish and many parting kisses; but in face of his enemies, and in hearing of the cries which burst from the ranks demanding her death by fire as a murderess and harlot, the whole heroic and passionate spirit of the woman represented by her admirers as a spiritless imbecile flamed out in responsive threats to have all the men hanged and crucified in whose power she now stood helpless and alone. She grasped the hand of Lord Lindsay as he rode beside her, and swore "by this hand" she would "have his head for this." In Edinburgh she was received by a yelling mob, which flaunted before her at each turn a banner representing the corpse of Darnley, with her child beside it, invoking on his knees the retribution of divine justice.

From the violence of a multitude, in which women of the worst class were more furious than the men, she was sheltered in the house of the provost, where she repeatedly showed herself at the window, appealing aloud with dishevelled hair and dress to the mercy which no man could look upon her and refuse. At nine in the evening she was removed to Holyrood, and thence to the port of Leith, where she embarked under guard, with her attendants, for the island castle of Lochleven. On the 20th a silver casket containing letters and French verses, miscalled sonnets, in the handwriting of the Queen, was taken from the person of a servant who had been sent by Bothwell to bring it from Edinburgh to Dunbar. Even in the existing versions of the letters, translated from the lost originals and retranslated from this translation of a text which was probably destroyed in 1603 by order of King James on his accession to the English throne—even in these possibly disfigured versions, the fiery pathos of passion, the fierce and piteous fluctuations of spirit between love and hate, hope and rage and jealousy, have an eloquence apparently beyond the imitation or invention of art.

Three days after this discovery Lord Lindsay, Lord Ruthven, and Sir Robert Melville were despatched to Lochleven, there to obtain the Queen's signature to an act of abdication in favor of her son, and another appointing Murray regent during his minority. She submitted, and a commission of regency was established till the return from France of Murray, who, on August 15th, arrived at Lochleven with Morton and Athol. According to his own account the expostulations as to her past conduct which preceded his admonitions for the future were received with tears, confessions, and attempts at extenuation or excuse; but when they parted next day on good terms, she had regained her usual spirits. Nor from that day forward had they reason to sink again, in spite of the close keeping in which she was held, with the daughters of the house for bedfellows. Their mother and the Regent's, her father's former mistress, was herself not impervious to her prisoner's lifelong power of seduction and subjugation. Her son George Douglas fell inevitably under the charm. A rumor transmitted to England went so far as to assert that she had proposed him to their common half-brother Murray as a fourth husband for herself; a later tradition represented her as the mother of a child by him. A third report, at least as improbable as either, asserted that a daughter of Mary and Bothwell, born about this time, lived to be a nun in France.

It is certain that the necessary removal of George Douglas from Lochleven enabled him to devise a method of escape for the prisoner on March 25, 1568, which was frustrated by detection of her white hands under the disguise of a laundress. But a younger member of the household, Willie Douglas, aged eighteen, whose devotion was afterward remembered and his safety cared for by Mary at a time of utmost risk and perplexity to herself, succeeded on May 2d in assisting her to escape by a postern gate to the lake-side, and thence in a boat to the mainland, where George Douglas, Lord Seton, and others were awaiting her. Thence they rode to Seton's castle of Niddry, and next day to Hamilton palace, round which an army of six thousand men was soon assembled, and whither the new French ambassador to Scotland hastened to pay his duty. The Queen's abdication was revoked, messengers were despatched to the English and French courts, and word was sent to Murray at Glasgow that he must resign the regency, and should be pardoned in common with all offenders against the Queen. But on the day when Mary arrived at Hamilton, Murray had summoned to Glasgow the feudatories of the crown, to take arms against the insurgent enemies of the infant King.

On the 13th of May the battle or skirmish of Langside determined the result of the campaign in three-quarters of an hour. Kirkaldy of Grange, who commanded the Regent's cavalry, seized and kept the place of vantage from the beginning, and at the first sign of wavering on the other side shattered at a single charge the forces of the Queen with a loss of one man to three hundred. Mary fled sixty miles from the field of her last battle before she halted at Sanquhar, and for three days of flight, according to her own account, had to sleep on the hard ground, live on oatmeal and sour milk, and fare at night like the owls, in hunger, cold, and fear.

On the third day from the rout of Langside she crossed the Solway, and landed at Workington in Cumberland, May 16, 1568. On the 20th Lord Scrope and Sir Francis Knollys were sent from court to carry messages and letters of comfort from Elizabeth to Mary at Carlisle. On June 11th Knollys wrote to Cecil at once the best description and the noblest panegyric extant of the Queen of Scots—enlarging, with a brave man's sympathy, on her indifference to form and ceremony, her daring grace and openness of manner, her frank display of a great desire to be avenged of her enemies, her readiness to expose herself to all perils in hope of victory, her delight to hear of hardihood and courage, commending by name all her enemies of approved valor, sparing no cowardice in her friends, but above all things athirst for victory by any means at any price, so that for its sake pain and peril seemed pleasant to her, and wealth and all things, if compared with it, contemptible and vile.

Mary was held a prisoner in England for seventeen years. In 1585 she was accused of favoring Anthony Babington's plot against the life of Elizabeth, her captor. Anthony Babington, in his boyhood a ward of Shrewsbury, resident in the household at Sheffield castle, and thus subjected to the charm before which so many victims had already fallen, was now induced to undertake the deliverance of the Queen of Scots by the murder of the Queen of England. It is maintained by those admirers of Mary who assume her to have been an almost absolute imbecile, gifted with the power of imposing herself on the world as a woman of unsurpassed ability, that, while cognizant of the plot for her deliverance by English rebels and an invading army of foreign auxiliaries, she might have been innocently unconscious that this conspiracy involved the simultaneous assassination of Elizabeth. In the conduct and detection of her correspondence with Babington, traitor was played off against traitor, and spies were utilized against assassins, with as little scruple as could be required or expected in the diplomacy of the time.

As in the case of the casket letters, it is alleged that forgery was employed to interpolate sufficient evidence of Mary's complicity in a design of which it is thought credible that she was kept in ignorance by the traitors and murderers who had enrolled themselves in her service—that one who pensioned the actual murderer of Murray and a would-be murderer of Elizabeth was incapable of approving what her keen and practised intelligence was too blunt and torpid to anticipate as inevitable and inseparable from the general design. In August the conspirators were netted, and Mary was arrested at the gate of Tixall Park, whither Paulet had taken her under pretence of a hunting-party. At Tixall she was detained till her papers at Chartley had undergone thorough research. That she was at length taken in her own toils, even such a dullard as her admirers depict her could not have failed to understand; that she was no such dastard as to desire or deserve such defenders, the whole brief course of her remaining life bore consistent and irrefragable witness.