1st. I saw you scrape from the skin of an ague patient sweat and epithelium with the spores and the full grown plants of the Gemiasma verdans.
2d. I saw you take the sputa of a ague patient and demonstrate the spores and sporangia of the Gemiasma verdans.
3d. I saw you take the urine of a female patient suffering from ague (though from motives of delicacy I did not see the urine voided--still I believe that she did pass the urine, as I did not think it necessary to insult the patient), and you demonstrated to me beautiful specimens of Gemiasma rubra. You said it was not common to find the full development in the urine of such cases, but only in the urine of the old severe cases. This was a mild case.
4th. I saw you take the blood from the forearm of an ague patient, and under the microscope I saw you demonstrate the gemiasma, white and bleached in the blood. You said that the coloring matter did not develop in the blood, that it was a difficult task to demonstrate the plants in the blood, that it required usually a long and careful search of hours sometimes, and at other times the plants would be obtained at once.
When I had fully comprehended the significance of the experiments I was filled with joy, and like the converts in apostolic times I desired to go about and promulgate the news to the profession. I did so in many places, notably in New York city, where I satisfactorily demonstrated the plants to many eminent physicians at my room at the Fifth Avenue Hotel; also before a medical society where more than one hundred persons were present. I did all that I could, but such was the preoccupation of the medical gentlemen that a respectful hearing was all I got. This is not to be wondered at, as it was a subject, now, after the lapse of nearly a decade and a half, quite unstudied and unknown. After this I studied the plants as I had opportunity, and in 1877 made a special journey to Long Island, N.Y., for the purpose of studying the plants in their natural habitat, when they were in a state of maturity. I have also examined moist soils in localities where ague is occasionally known, with other localities where it prevails during the warm months.
Below I give the results, which from convenience I divide into two parts: 1st. Studies of the ague plants in their natural habitat. 2d. Studies of the ague plants in their unnatural habitat (parasitic). I think one should know the first before attempting the second.
First--Studies to find in their natural habitat the palmellæ described as the Gemiasma rubra, Gemiasma verdans, Gemiasma plumba, Gemiasma alba, Protuberans lamella.
Second--Outfit.--Glass slides, covers, needles, toothpicks, bottle of water, white paper and handkerchief, portable microscope with a good Tolles one inch eyepiece, and one-quarter inch objective.
Wherever there was found on low, marshy soil a white incrustation like dried salt, a very minute portion was removed by needle or toothpick, deposited on a slide, moistened with a drop of water, rubbed up with a needle or toothpick into a uniformly diffused cloud in and through the water. The cover was put on, and the excess of water removed by touching with a handkerchief the edge of the cover. Then the capillary attraction held the cover in place, as is well known. The handkerchief or white paper was spread on the ground at my feet, and the observation conducted at once after the collection and on the very habitat. It is possible thus to conduct observations with the microscope besides in boats on ponds or sea, and adding a good kerosene light in bed or bunk or on lounge.
August 11, 1877.--Excursion to College Point, Flushing, Long Island: