Now the amœboid state is a notable phenomenon throughout the monads as precursive of striking change. It appears to subserve the purpose of the more facile acquisition and digestion of food at a crisis. And this augmented the difficulty of discovering further change; and only persistent effort enabled us to discover that with comparative rareness there appeared a form in an amœboid state that was unique. It was a condition chiefly confined to the caudal end, the sarcode having became diffluent, hyaline, and intensely rapid in the protrusion and retraction of its substance, while the nuclear body becomes enormously enlarged. These never appear alone; forms in a like condition are diffused throughout the fluid, and may swim in this state for hours. Meanwhile, the diffluence causes a spreading and flattening of the sarcode and swimming gives place to creeping, while the flagella violently lash. In this condition two forms meet by apparent accident, the protrusions touch, and instant fusion supervenes. In the course of a few seconds there is no disconnected sarcode visible, and in five to seven minutes the organism is a union of two of the organisms, the swimming being again resumed, the flagella acting in apparent concert. This may continue for a short time, when movement begins to flag and then ceases. Meanwhile, the bodies close together, and the eyenots or vacuoles melt together, the two nuclei become one and disappear, and in eighteen hours the entire body of "either has melted into other," and a motionless, and for a time irregular, sac is left. This now becomes smooth, spherical, and tight, being fixed and motionless. This is a typical process; but the mingled weariness and pleasure realized in following such a form without a break through all the varied changes into this condition is not easily expressed.
But now the utmost power of lenses, the most delicate adjustment of light, and the keenest powers of eyesight and attention must do the rest. Before the end of six hours the delicate glossy sac opens gently at one place, then there streams out a glairy fluid densely packed with semi-opaque granules, just fairly visible when their area was increased six millions of times, and this continued until the whole sac was empty and its entire contents diffused. To follow with our utmost powers these exquisite specks was an unspeakable pleasure, a group seen to roll from the sac, when nearly empty, were fixed and never left. They soon palpably changed by apparent swelling or growth, but were perfectly inactive; but at the end of three hours a beaked appearance was presented. Rapid growth set in, and at the end of another hour, how has entirely baffled us, they acquired flagella and swam freely; in thirty-five minutes more they possessed a nucleus and rapidly developed, until at the end of nine hours after emission a sporule was followed to the parent condition and left in the act of fission. In this way, with what difficulties I need not weary you, a complete life-cycle was made out.
And now I will invite your attention to the developmental history of the most minute of the six forms we studied. In form it is a long oval, it is without visible structure or differentiation within, and is possessed of only a single flagellum. Its utmost length is the 1/5000 of an inch. Its motion is continuous in a straight line, and not intensely rapid, nor greatly varied, being wholly wanting in curves and dartings. The copiousness of its increase was, even to our accustomed eyes, remarkable in the extreme, but the reason was discovered with comparative ease. Its fission was not a division into two, but into many. The first indication of its approach in following this delicate form was the assumption rapidly of a rounder shape. Then followed an amœboid and uncertain form, with an increased intensity of action which lasted a few moments, when lassitude supervened, then perfect stillness of the body, which is now globular in form, while the flagellum feebly lashed, and then fell upon and fused with the substance of the sarcode. And the result is a solid, flattened, homogeneous ball of living jelly.
To properly study this in its further changes, a power of from three to four thousand diameters must be used, and with this I know of few things in the whole range of minute beauty more beautiful than the effect of what is seen. In the perfectly motionless flattened sphere, without the shimmer of premonition and with inconceivable suddenness, a white cross smites itself, as it were, through the sarcode. Then another with equal suddenness at right angles, and while with admiration and amazement one for the first time is realizing the shining radii, an invisible energy seizes the tiny speck, and fixing its center, twists its entire circumference, and endows it with a turbined aspect. From that moment intense interior activity became manifest. Now the sarcode was, as it were, kneading its own substance, and again an inner whirling motion was visible, reminding one of the rush of water round the interior of a hollow sphere on its way to a jet or fountain. Deep fissures or indentations showed themselves all over the sphere; and then at the end of ten or more minutes all interior action ceased, and the sphere had segmented into a coiled mass. There was no trace of an investing membrane; the constituent parts were related to each other simply as the two separating parts of an ordinary fission; and they now commenced a quick, writhing motion like a knot of eels, and then, in the course of from seven to thirty minutes, separated, and fully endowed with flagella swam freely away, minute but perfect forms, which by the rapid absorption of pabulum attained speedily to the parent size.
It is characteristic of this group of organic forms that multiplication by self-division is the common and continuous method of increase. The other and essential method was comparatively rare and always obscure. In this instance, on the first occasion the continuous observation of the same "field" for five days failed to disclose to us any other method of increase but this multiple-fission, and it was only the intense suggestiveness of past experience that kept us still alert and prevented us from inferring that it was the only method. But eventually we perceived that while this was the prevailing phenomenon, there were scattered among the other forms of the same monad larger than the rest, and with a singular granular aspect toward the flagellate end. It may be easily contrasted with the normal or ordinary form. Now by doggedly following one of these through all its wanderings a wholly new phase in the morphology of the creature was revealed. This roughened or granular form seized upon and fastened itself to a form in the ordinary condition. The two swam freely together, both flagella being in action, but it was shortly palpable that the larger one was absorbing the lesser. The flagellum of the smaller one at length moved slower, then sluggishly, then fell upon the sarcode, which rapidly diminished, while the bigger form expanded and became vividly active until the two bodies had actually fused into one. After this its activity diminished, in a few minutes the body became quite still, leaving only a feeble motion in the flagellum, which soon fell upon the body-substance and was lost. All that was left now was a still spheroidal glossy speck, tinted with a brownish yellow. A peculiarity of this monad is the extreme uncertainty of the length of time which may elapse before even the most delicate change in this sac is visible. Its absolute stillness may continue for ten or more hours. During this time it is absolutely inert, but at last the sac—for such it is—opens gently, and there is poured out a brownish glairy fluid. At first the stream is small, but at length its flow enlarges the rift in the cyst, and the cloudy volume of its contents rolls out, and the hyaline film that inclosed it is all that is left.
The nature of the outflow was like that produced by the pouring of strong spirit into water. But no power that we could employ was capable of detecting a granule in it. To our most delicate manipulation of light, our finest optical appliances, and our most riveted attention, it was a homogeneous fluid and nothing more. This for a while baffled and disturbed us. It lured us off the scent. We inferred that it might possibly be a fertilizing fluid, and that we must look in other directions for the issue. But this was fruitless, and we were driven again to the old point, and having once more obtained the emitted fluid, determined to fix a lens magnifying 5,000 diameters upon a clear space over which the fluid had rolled, and near to the exhausted sac, and ply our old trade of watching with unbroken observation.
The result was a reward indeed. At first the space was clear and white, but in the course of a hundred minutes there came suddenly into view the minutest conceivable specks. I can only compare the coming of these to the growth of the stars in a starless space upon the eye of an intense watcher in a summer twilight. You knew but a few minutes since a star was not visible there, and now there is no mistaking its pale beauty. It was so with these inexpressibly minute sporules; they were not there a short time since, but they grew large enough for our optical aids to reveal them, and there they were. Such a field after one hour's watching I present to you. And here I would remark that these delicate specks were unlike any which we saw emerge directly from the sac as granules. In that condition they were always semi-opaque, but here they were transparent, and a brown yellow, the condition always sequent upon a certain measure of growth.
To follow these without the loss of an instant's vision was pleasure of the highest kind. In an hour and ten minutes from their first discovery they had grown to oval points. In one hour more the specks had become beaked and long. And this pointed end was universally the end from which the flagellum emerged. With the flagellum comes motion, and with that abundant pabulum, and therefore rapid growth. But when motion is attained we are compelled to abandon the mass and follow one in all its impetuous travels in its little world; and by doing so we are enabled to follow the developed speck into the parent condition and size, and not to leave it until it had, like its predecessors, entered on and completed its wonderful self-division by fission.
It becomes then clearly manifest that these organisms, lowly and little as they are, arise in fertilized parental products. There is no more caprice in their mode of origin than in that of a crustacean or a bird. Their minuteness, enormous abundance, and universal distribution is the explanation of their rapid and practically ubiquitous appearance in a germinating and adult condition. The presence of putrefiable or putrescent matter determines at once the germination of the always-present spore. But a new question arises. These spores are definite products. In the face of some experimental facts one was tempted to inquire: Have these spores any capacity to resist heat greater than the adults? It was not easy to determine this question. But we at length were enabled to isolate the germs of seven separate forms, and by means of delicate apparatus, and some twelve months of research, to place each spore sac in an apparatus so constructed that it could be raised to successive temperatures, and without any change of conditions examined on the stage of the microscope.
In this way we reached successive temperatures higher and higher until the death point—the point beyond which no subsequent germination ever occurred—was reached in regard to each organism. The result was striking. The normal death point for the adult was 140° F. One of the monads emitted from its sac minute mobile specks—evidently living bodies—which rapidly grew. These we always destroyed at a temperature of 180° F. Three of the sacs emitted spores that germinated at every temperature under 250° F. Two more only had their power of germination destroyed at 260° F. And one, the least of all the monad forms, in a heat partially fluid and partially dry, at all points up to 300° F. But if wholly in fluid it was destroyed at the point of 290° F. The average being that the power of heat resistance in the spore was to that of the adult as 11 to 6. From this it is clear that we dare not infer spontaneous generation after heat until we know the life-history of the organism.