Besides the foregoing objections the surgical instruments decompose the peroxide, hence, if an operation is to be performed, the surgeon uses some other antiseptic during the procedure, and is apt to continue the application of the same antiseptic in the subsequent dressings.
Nevertheless, the satisfactory results which I have obtained at the Pasteur Institute of New York with peroxide of hydrogen, in the treatment of wounds resulting from deep bites, and those which I have observed at the French clinic of New York, in the treatment of phagedenic chancres, varicose ulcers, parasitic diseases of the skin, and also in the treatment of other affections caused by germs, justify me in adding my statement as to the value of the drug.
But, it is not from a clinical standpoint that I now direct attention to the antiseptic value of peroxide of hydrogen. What I now wish is merely to give a full report of the experiments which I have made on the effects of peroxide of hydrogen upon cultures of the following species of pathogenic microbes: Bacillus anthracis, bacillus pyocyaneous, the bacilli of typhoid fever, of Asiatic cholera, and of yellow fever, streptococcus pyogenes, micro-bacillus prodigiosus, bacillus megaterium, and the bacillus of osteomyelites.
The peroxide of hydrogen which I used was a 3.2% solution, yielding fifteen times its volume of oxygen; but this strength reduced to about 1.5%, corresponding to about eight volumes of oxygen, by adding the fresh culture containing the microbe upon which I was experimenting. I have also experimented upon old cultures loaded with a large number of the spores of the bacillus anthracis. In all cases my experiments were made with a few cubic centimetres of the culture in sterilized test-tubes, in order to obtain accurate results.
The destructive action of peroxide of hydrogen, even diluted in the above proportions, is almost instantaneous. After a contact of a few minutes, I have tried to cultivate the microbes which were submitted to the peroxide, but unsuccessfully, owing to the fact that the germs had been completely destroyed.
My next experiments were made on the hydrophobic virus in the following manner:
I mixed with sterilized water a small quantity of the medulla that had been taken from a rabbit that had died of hydrophobia, and to this mixture added a small quantity of peroxide of hydrogen. Abundant effervescence took place, and, as soon as it ceased, having previously trephined a rabbit, I injected a large dose of the mixture under the dura mater. Slight effervescence immediately took place, and lasted a few moments, but the animal was not more disturbed than when an injection of the ordinary virus is given. This rabbit is still alive, two months after the inoculation.
A second rabbit was inoculated with the same hydrophobic virus, which had not been submitted to the action of the peroxide, and this animal died at the expiration of the eleventh day, with the symptoms of hydrophobia.