As might be expected, Mary's enemies accuse her of a criminal degree of inactivity after the death of her husband. But what could she do? Who were the murderers? No one could tell. The whole affair was then involved in impenetrable mystery. Her chief officers of justice, Huntly the chancellor, and Argyll the lord-justice, were both in the plot; Bothwell, the sheriff of the county, on whom should devolve the pursuit and arrest of the criminal, had taken an active share in the perpetration of the murder, and Maitland, the secretary, who had first proposed to get rid of Darnley, was probably the most guilty of all. In a memorial afterward addressed by Mary to the different European courts, she thus describes the situation: "Her majesty could not but marvel at the little diligence they used, and that they looked at one another as men who wist not what they say or do."

And now calumny ran riot. Slanderous tongues and pens were busy. Since Mary had dismissed the insolent Randolph from her court, Elizabeth had maintained no ambassador there, so that the usual official espionage could not be carried on. Instead thereof, Sir William Drury, stationed on the Scotch border, transmitted day by day a current of scandalous stories. Mary was a woman, and her enemies might effect by slander what they could not accomplish by force. Then, too, a bigoted religious prejudice made the work easy. No matter, says our author, what was the nature of the accusation against a Catholic queen; so long as it was boldly made and frequently repeated, it was sure to gain a certain amount of credit in the end. Here follows, in Mr. Hosack's pages, an able presentation of contemporary testimony going to show the falsehood of the accusations that the queen was at this time on a footing of intimate understanding with Bothwell. Under the circumstances his trial was, of course, a farce.

The most powerful men in Scotland were his associates in guilt. One of his noble accomplices in the murder rode by his side to the Talbooth. Another accomplice, the Earl of Argyll, hereditary lord-justice, presided at the trial; and the Earl of Caithness, a near connection of Bothwell by marriage, was foreman of the jury. The parliament which met soon after did little, besides passing the Act of Toleration, but enact statutes confirming Maitland, Huntly, Morton, and Murray in their titles and estates. As we have seen, this was precisely the main object sought by these men in the murder of Darnley, an object passed over in silence by most historians, and not understood by others. Their common interest in his death was the strongest bond of union among the noble assassins. If Darnley had lived, he would have prevented the confirmation of these grants; for he had made significant threats on that subject, especially as to the gifts to Murray. Murray and the others wanted the lands and titles. They obtained them. Bothwell had his own designs, and these were insolent in their ambition. He wanted the queen's hand in marriage as a step to the throne. It was but just that his companions should help him as he had aided them. On the evening of the day on which parliament rose, (April 19th,) Both well gave an entertainment at a tavern in Edinburgh to a large party of the nobility. After wine had circulated freely, he laid before his guests a bond for their signatures. This document recited that it was prejudicial to the realm that the queen should remain a widow; and it recommended him, (Bothwell,) a married man, as the fittest husband she could obtain among her subjects. With a solitary exception—the Earl of Eglinton—all the lords present signed this infamous bond, and thereby bound themselves to "further advance and set forward the said marriage," and to risk their lives and goods against all who should seek to hinder or oppose it. It is claimed by Mr. Froude that his special saint, "the noble and stainless Murray," did not sign this bond; but it is now made plain that he did. Meantime calumny had free scope, and no invention was too gross for belief by many, if it but carried with it some injury to Mary's reputation. Thus, she is accused of journeying to Stirling for the express purpose of poisoning her infant son. Poor Marie Antoinette in after years, as we know, was accused of something worse than taking the life of her child. The answer of these two Catholic queens, great in their sufferings, and grand in their resignation, was, in each case, an eloquent burst of nature and queenly dignity. "The natural love," said Mary Stuart, "which the mother bears to her only bairn is sufficient to confound them, and needs no other answer." She afterward added, that all the world knew that the very men who now charged her with this atrocious crime had wronged her son even before his birth; for they would have slain him in her womb, although they now pretended in his name to exercise their usurped authority.

On the 23d of April, while travelling from Linlithgow to Edinburgh, with a few attendants, the queen was stopped by Bothwell, at the head of one thousand horse. Bothwell rode up, caught her bridle-rein, and assured her that "she was in the greatest possible danger," and forthwith escorted her to one of her own castles, Dunbar. Here she was kept a prisoner. Melville, who accompanied her, was sent away, having heard Bothwell boast that he would marry the queen, even "whether she would herself or not." No woman was allowed near her but Bothwell's sister.

Although our readers are familiar with the horrible story, the best account of it is, after all, Mary's own simple and modest narrative of the abominable outrage. It is found in Keith, vol. ii. p. 599, and in Hosack, p. 313. After referring to the great services and unshaken loyalty of Bothwell, she says that, previous to her visit to Stirling, he had made certain advances, "to which her answer was in no degree correspondent to his desire;" but that, having previously obtained the consent of the nobility to the marriage, he did not hesitate to carry her off to the castle of Dunbar; that when she reproached him for his audacity, he implored her to attribute his conduct to the ardor of his affection, and to condescend to accept him as her husband, in accordance with the wishes of his brother nobles; that he then, to her amazement, laid before her the bond of the nobility, declaring that it was essential to the peace and welfare of the kingdom that she should choose another husband, and that, of all her subjects, Bothwell was best deserving of that honor; that she still, notwithstanding, refused to listen to his proposals, believing that, as on her former visit to Dunbar, an army of loyal subjects would speedily appear for her deliverance; but that, as day after day passed without a sword being drawn in her defence, she was forced to conclude that the bond was genuine, and that her chief nobility were all in league with Bothwell; and finally, that, finding her a helpless captive, he assumed a bolder tone, and "so ceased he never till, by persuasion and importunate suit, accompanied not the less by force, he has finally driven us to end the work begun." Forced to marry Bothwell Mary was, to all who saw her, an utterly wretched woman, and longed only for death. The testimony on this point is very ample, and her behavior at this crisis of her history, concludes Mr. Hosack, can only be explained by her rooted aversion to a marriage which was forced upon her by the daring ambition of Bothwell and the matchless perfidy of his brother nobles.

But already a fresh plot was on foot. Melville wrote to Cecil concerning it, on the 7th of May; and on the following day, Kirkaldy of Grange sent to the Earl of Bedford a letter intended for Elizabeth's eye. Kirkaldy, the Laird of Grange, an ardent Protestant, who, at the age of nineteen, was one of the men who murdered Cardinal Beaton, enjoyed among his fellow-nobles the reputation of being a man of honor, and the best and bravest soldier in Scotland. He advised Bedford of the signing of a "bond" by "the most part of the nobility," one head of which was, "to seek the liberty of the queen, who is ravished and detained by the Earl of Bothwell;" another, "to pursue them that murdered the king." The letter concludes by asking Elizabeth's aid and support for "suppressing of the cruel murtherer Bothwell." But Elizabeth had lost not only much money, but all credit for veracity, by her last interference in Scottish affairs, and refused to have any thing to do with this plot.

For three weeks after her marriage the queen remained at Holyrood; the prisoner, to all appearance, rather than the wife of Bothwell. She was continually surrounded with guards; and the description of her situation given by Melville, who was at court at the time, agrees entirely with that of the French ambassador. Not a day passed, he says, in which she did not shed tears; and he adds that many, even of Bothwell's followers, "believed that her majesty would fain have been quit of him." The insurgent leaders—Morton, Maitland, and Hume—were busy, and soon in the field with their forces. Bothwell raised a small levy to oppose them, and the two armies met at Carberry Hill on the 15th of June, 1567, exactly one month after the marriage. There was no fighting. Dangerous as it was, Mary preferred to trust herself to the rebel lords than to remain with Bothwell. She received their pledge—that, in case she would separate herself from Bothwell, they were ready "to serve her upon their knees, as her most humble and obedient subjects and servants"—through Kirkaldy of Grange, the only man among them whose word she would take. They kept their pledge as they usually observed such obligations. What followed is too horrible to dwell upon. It is wonderful that any human being could have lived through the physical exhaustion, the insults, and the brutal treatment this poor woman was subjected to during the next two days. The people of Edinburgh grew indignant; and Kirkaldy of Grange swore the lords should not violate their promises. But they quieted him by showing a forged letter of the queen to Bothwell. It was not the first time some among them had forged Mary's signature. With every circumstance of force and brutality, Mary was then imprisoned in Lochleven, whose guardian was the mother of the bastard Murray.

And now, while the friends of Mary, numerous as they were, remained irresolute and inactive, the dominant faction made the most strenuous efforts to strengthen itself. In the towns, where its strength chiefly lay, and especially in Edinburgh, says Mr. Hosack, the Protestant preachers rendered the most valuable aid. By indulging in furious invectives against the queen, and charging her directly with the murder, they prepared their hearers for the prospect of her speedy deposition, and the establishment of a regency in the name of the infant prince. It is clear that Murray was not forgotten by his friends the preachers.

Strange as it may appear, there can be but little doubt that Elizabeth was sincerely indignant on hearing of the outrageous treatment of Mary by the lords. In her whole history, she never appeared to so much advantage as a woman and a queen. She would not stand tamely by, she said, and see her cousin murdered; and if remonstrances proved ineffectual, she would send an army to chastise and reduce them to obedience. Such conduct, and her messages to Mary while a prisoner at Lochleven, no doubt inspired the Scottish queen with the fatal confidence which induced her, a few months afterward, to seek refuge in England. Unfortunately for Elizabeth, and perhaps more unfortunately for Mary, the Queen of England's reputation for duplicity was now so well established that no one but her own ministers believed she was now sincere. Maitland, for the Scotch nobles, plainly told Elizabeth's ambassador that, after what had occurred in times past, "they could place no reliance on his mistress;" and the King of France said to Sir Henry Norris, "I do not greatly trust her." Meantime, the ministers daily denounced Mary as a murderess in their sermons, and demanded that she should be brought to justice like an ordinary criminal. Elizabeth's ambassador tried to induce the confederate lords to restrain the savage license of the preachers; but we cannot doubt, says Mr. Hosack, that they were secretly encouraged by their noble patrons to prepare the minds of the people for the deposition, if not for the murder, of the queen. Throgmorton's opinion was that, but for his presence in Scotland, she would have been sacrificed to the ambition and the bigotry of her subjects.

Still a prisoner at Lochleven, Mary had to suffer the brutality of the ruffian Lindsay, and the infamous hypocrisy of Mr. Froude's "stainless Murray," who, with money in both pockets from France and England, now came, with characteristic deceit, to defraud his sister of her crown. Mr. Hosack thus estimates his performance: