A striking effect of the low temperature was noticed in the effect on the hatching of the eggs. Many of the females taken at the mouth of the cave carried eggs or recently hatched young, while none of those taken in the other localities were found with young at all. I believe I found young crayfish which had but recently left the female in the vegetation near Boiling Springs, in the James river.

In the table following, the distribution by river systems of the different species is given. For convenience, the rivers of the state from which material has been reported have been arranged as follows:

The tributaries of any stream are arranged in order, beginning with the lower and passing towards the upper portion of the stream. Those tributaries emptying outside the state are designated by an asterisk.

When it is impossible to determine from which of two or more streams a lot of material is reported, as is often the case when the localities given is a country traversed or drained by two rivers, or a town situated on some large stream, or where two streams join, it is reported from each, with the catalogue number followed by a question mark. Of course, in the greater number of these cases, the species will be found to occur in greater or less numbers in each locality. The one thing to be desired is, that collectors would furnish full data with their material.

Whenever possible, the name of the stream from which material was taken is given. When this is not possible, the term “tributary” is used. As a general rule, the tributaries are streams emptying directly into the river under which they are placed, and the only exceptions to this, I believe, are the tributaries of the Arkansas arising in the southeastern corner of the state. Stagnant ponds in the region drained by a stream have been classed as tributaries, even through they have no direct connection.

The above method of classifying the water systems of the state will, very possibly, be found not the best for a final arrangement, but for a preliminary classification—and nothing more than a preliminary arrangement can be hoped for at present—it seems quite satisfactory.