Sections should be made (a) longitudinally through the cardiac end to show the transition from the œsophageal to the gastric mucous membrane, (b) from a portion of the greater curvature, (c) longitudinally through the pyloric valve.
Eosine and hæmatoxyline form the best stain for the alimentary canal.
Intestine.—Prepare in the same way as the stomach. Make sections from (a) the upper part of the duodenum to show Brunner’s glands, (b) the ileum, (c) a Peyer’s patch, (d) the vermiform appendix, (e) the colon.
Liver.—Make an injection of one specimen with carmine and gelatin (p. [120]). Harden in methylated spirit. Others should be hardened in Müller’s fluid and stained in the usual way.
Kidney, supra-renal, and pancreas.—Same preparation as for liver.
Spleen.—Harden in Müller’s fluid.
Mount one section unstained. Shake another up with water in a test tube to shew the structure of the pulp. Stain others in eosine and hæmatoxyline.
Bladder.—Must be removed and pinned out immediately after death, as otherwise the epithelium will be macerated off. Consequently it must be taken from an animal, as a cat. Harden in osmic acid. Cut in celloidin as the coats are very apt to become detached.
Penis and testis.—Readily obtained from dog, cat, or rat.
Stain with eosine and hæmatoxyline.