The amount of caffein received during the entire experimental period was 9.2223 grams, or an average per day approximately of 80 mg per kilo, and therefore 10 per cent more than dog No. 30 received. It will be observed that the appetite in dog No. 32 was likewise uniformly good, and that he received a very high protein diet which was also of a very high calorific value.
Autopsy (dog 32).—Stomach presented a severe inflammation of the mucosa, especially in the fundus and pyloric portions. The gastritis was more marked in pyloric portion, and the inflammatory condition extended along the whole course of small intestines, which presented numerous hemorrhagic areas, and a thick catarrhal exudate on the mucosa. The large intestine contained quite a large number of parasites, probably round worms. The liver was enlarged and the gall cyst well filled. The spleen was also considerably engorged, kidneys appeared normal, other organs all appeared normal.
Dog 31. Black spaniel, male.
This dog had been under observation one month previous to the experiments with caffein. The usual initial dose was then administered for nine days. There were no signs of local irritation when the drug was given subcutaneously, but symptoms of toxicity were present. These disappeared, however, when the drug was administered by mouth. The dose was therefore increased to 75 mg per kilo. This, as will be seen, proved fatal within six hours. High nitrogen diet, same as No. 30.
November 3: Weight 10.250 kilos.
November 10: Weight, 10.25 kilos.
November 20: Weight, 10.30 kilos.
December 1: Put in cage.
December 6, 7, 8, 9: Weight, 10.20 kilos; received 26 cc 2 per cent caffein subcutaneously, site of injection normal.