July 17: 3.30 p. m., 10 cc (0.125 gram per kilo) of 2 per cent caffein given by mouth through stomach tube.

July 18: Under observation all day, no symptoms.

From the results of the experiments of this series it appears that 0.15 gram caffein per kilo may be fatal within a few hours after its administration, even if the drug is mixed with a moderate amount of meat. Experiments 87 and 92 show, however, that this amount may be borne by some individuals without any serious consequences, as the cats were under observation for some time after they received caffein, and no untoward symptoms were noticed in either of them during this time. It may be remarked that cat No. 92 vomited shortly after it received caffein. It is practically certain, therefore, that this amount of caffein in proportion to the weight of the animal will in the great majority of cases prove fatal, and perhaps in a smaller percentage of individuals it is surely toxic if it does not escape absorption. Smaller doses may cause irritability in some individuals, but symptoms referable to nervous symptoms of muscles were absent, as in experiments Nos. 93 and 100. The minimum fatal dose of caffein for the cat when given by mouth is, therefore, 0.15 gram per kilo.

Table 9.—Subcutaneous injection; cats.

SERIES A.
Number. Weight.Caffein per kiloSymptoms.Duration of life.
Grams. Gram.
4 1,440 0.30 65 minutesOver 29 hours.
5 1,396 .30  About 2 hours.
SERIES B.
3 2,854 0.25  30 minutes.
6 1,645 .243 Few minutes 1 hour 30 minutes.
8 1,735 .25  30 minutes. 1 hour.
9 1,960 .25  1 hour 1 hour 45 minutes.
12 1,185 .20  3 hours Less than 18 hours.
14 1,855 .20  1 hour 20 minutes Do.
15 2,145 .20  40 minutes. Do.
19 1,100 .236 15 minutes Do.
20 790 .25  4 hours 30 minutes.
SERIES C.
24 1,300 0.153 1 hour Survived.
17 2,620 .15  15 minutes Do.
23 1,645 .15  1 hour 10 minutes.
7 1,285 .155 20 minutes 1 hour.
9 2,285 .14  65 minutes.
SERIES D.
13 730 0.139 Restlessness
21 1,165 .138 None
25 965 .103 do.
26 1,605 .125 do.
27 1,625 .125 do.
28 2,335 .128 do. Received 2 doses: survived.
40 2,710 .129 do. Do.
41 1,785 .123 do. Do.
42 2,315 .112 do. Do.
38 2,325 .120 Mild Died after second dose.
SERIES E.
43 3,225 0.124 40 minutes.
48 3,050 .118 Died soon after.
47 4,220 .084 Survived.
247 4,250 .084 2 hours.

1 Pathological conditions. 2 Two days after first injection.

Table 10.—Injections into peritoneal cavity; cats.

SERIES A.
Number. Weight.Caffein per kiloSymptoms.Duration of life.
Grams. Gram.
99 3,000 0.100 Mild Survived.
98 4,100 .100 do.1 Do.
93 1,450 .137 Very mild Do.
87 2,615 .145 do. Do.
97 505 .200 None 5 days.
96 575 .139 do. 6 days.
95 860 .200 15 minutes 30 minutes.
94 790 .200 Diarrhea Survived.
10 2,970 .200 1 hour.
16 2,420 .183 30 minutes.

1 In few minutes.