C. TOXICITY EXPERIMENTS
By the Referee
TECHNIC
White rats were injected hypodermically with chlorlyptus or with eucalyptus oil, diluted with olive oil in the ratio of 1:4. The larger doses were divided between two or more sites of injection.
DETAILED PROTOCOLS
Hypodermic injections in white rats. Drugs diluted with 3 parts of olive oil. Doses are given as cubic centimeters of pure drug per kilogram of rat.
A. EUCALYPTUS SERIES
Experiment 1.—1.25 c.c.; injected VII.9.19: Active; walks about. No depression at any time. VII.10.19. Appears normal.
Experiment 2.—2.5 c.c.; injected VI.30.19: Quiet—not very depressed, reflexes good (six hours).
VII.1.19—Active—reflexes good, eats moderately.
VII.2.19—Animal acts normal—eats moderately, reflexes good; active (a.m.). Later in day, depressed.
VII.4.19—Died during night of VII.3.19.
Experiment 3.—3.75 c.c.; injected VI.24.19: Quiet; depressed; pain reflex diminished. Animal lay on ventral surface, not supported by legs. Will get on to feet very sluggishly if turned on side (twenty-four hours). Does not eat.
VI.26.19—Depressed slightly; pain reflex present.
VI.27.19—Fairly active; eats a little.
VI.28.19—Depressed.
Died during night of VI.29.19 (three days).
Experiment 4.—5 c.c.; injected VI.24.19: Quiet; markedly depressed (one hour). Does not get on feet when turned on side; ataxia well marked. Slight watery secretion in eyes. Reflexes diminished. Does not eat (twenty-four hours).
VI.26.19—Heart slowed and arrhythmic. Animal lies on side. Unable to walk; markedly depressed.
VI.27.19—Lies on side; does not eat. Died during night of VI.27.19 (three days).
Experiment 5.—6.25 c.c.; injected VI.24.19: Quiet; very markedly depressed. Heart and respiration greatly slowed. Lies on side; tears in eyes; does not eat (twenty-four hours).
VI.25.19—Temperature subnormal; cold to touch; tail stiffened and straight.
Died during night of VI.25.19 (one and one-half days).
Postmortem: Lungs congested. Liver pale in color. Spleen very dark red. Kidneys normal. Other organs normal.
B. CHLORLYPTUS EXPERIMENTS
Experiment 1.—1.56 c.c.; injected VI.24.19: Rather restless for an hour. Active during next four hours and following twenty-four. Eats well, reflexes good. Acts normal on VII.1.19 and since VI.26.19.
Experiment 2.—3.75 c.c.; injected VI.24.19: More quiet; active during next twenty-four hours. Reflex all right. Eats well; normal VII.1.19, since VI.26.19.
Experiment 3.—5 c.c.; injected VI.24.19: Quiet; defecation in four hours. Rather quiet for six hours. Eats well. Reflexes good; normal VII.1.19, since VI.26.19.
Experiment 4.—6.25 c.c.; injected VI.24.19: Quiet and breathing labored in four hours; active after twenty-four hours. Eats well. Somewhat depressed on VI.26.19; pain reflex present. On VI.26.19, eats well and fairly active. Active and eats, VI.27.19. Appears normal, VII.1.19.
Experiment 5.—8.75 c.c.; injected VI.30.19: Rather quiet during next two hours. Morning of VII.1.19, lies on stomach; quiet; does not eat very much. Pain reflexes good. VII.2.19, still depressed; does not eat. Appears normal, VII.3.19.
Experiment 6.—12.5 c.c.; injected VI.25.19: Quiet, but reflexes good; more quiet and depressed after several hours. Some loss of oil from wound. Died night of VI.25.19 (one day). Tail stiff. Temperature low.
Postmortem: Lungs markedly congested. Spleen and liver dark red. One kidney congested. Other viscera normal.
Experiment 7.—12.5 c.c.; injected VII.9.19: Quiet for one-half hour; 1.5 hours twitching of muscles of whole body, lies on side, ataxia present. Died night of VII.9.19 (one day).
Experiment 8.—18.75 c.c.; injected VI.25.19: Quiet; reflexes good (three hours). Some loss of oil. Depressed and turns on side (six hours). Died night of VI.25.19 (one day).
Postmortem: Lungs congested. Spleen and liver very dark red. Right kidney much darker red. Viscera normal.